PMID: 9192650Jun 24, 1997Paper

Construction and characterization of a reovirus double temperature-sensitive mutant

Proceedings of the National Academy of Sciences of the United States of America
Michael R RonerW K Joklik

Abstract

The infectious reovirus RNA system was used to construct a mutant with two temperature-sensitive (ts) lesions in genome segments M2 and S2, respectively. The double mutant is about 300 times more ts than either of its parents, which are about 1,500 and 170 times more ts than their wild-type parent reovirus ST3 strain Dearing. At 39 degrees C the double mutant is essentially unable to multiply. In spite of its striking temperature sensitivity, the double mutant elicits the formation of significant amounts of neutralizing antibodies in newborn mice. Possible mechanisms responsible for this are discussed, as is the significance of this double ts mutant in relation to current searches for safe and efficient vaccine strains.

References

Nov 1, 1977·The Journal of Experimental Medicine·H L Weiner, B N Fields
Dec 1, 1990·Virology·M R RonerW K Joklik
Jul 1, 1970·Virology·H J Zweerink, W K Joklik
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L W CashdollarW K Joklik
Jan 1, 1982·Journal of Virology·D Drayna, B N Fields
Mar 3, 1995·The Journal of Biological Chemistry·W K Joklik, M R Roner
Oct 1, 1994·Journal of Virology·M N Rozinov, B N Fields
Sep 1, 1993·Journal of Virology·P Lucia-JandrisB N Fields
Nov 1, 1993·Journal of Virology·P J BredenbeekS Schlesinger
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·M R RonerW K Joklik
Jan 1, 1996·Progress in Nucleic Acid Research and Molecular Biology·W K Joklik, M R Roner

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Citations

Apr 30, 2010·Journal of Virology·Cécile TroupinAntoine Garbarg-Chenon
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·M R Roner, W K Joklik
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Satoshi KomotoKoki Taniguchi
Nov 18, 2009·Expert Opinion on Biological Therapy·Diana J M Van Den WollenbergRob C Hoeben
Oct 20, 2006·Journal of Virological Methods·Michael R Roner, Christine Mutsoli
Dec 3, 2003·Journal of Evolutionary Biology·C L BurchK A Hanley
Sep 22, 2006·Virology·Michael R Roner, Bradley G Steele
Apr 21, 2007·Uirusu·Satoshi Komoto, Koki Taniguchi
Nov 21, 2007·The Journal of General Virology·Michael R Roner, Bradley G Steele
Dec 6, 2005·The Journal of Biological Chemistry·Wolfgang Karl Bill Joklik

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