Construction and characterization of a nonpigmented mutant of Porphyromonas gingivalis: cell surface polysaccharide as an anchorage for gingipains

Microbiology
Mikio ShojiKoji Nakayama

Abstract

A nonpigmented mutant of Porphyromonas gingivalis was constructed by using transposon mutagenesis. The mutant possessed the transposon DNA at the novel gene porR. Gene targeted mutagenesis revealed that porR was responsible for pigmentation. The porR gene shared similarities with genes of the degT family, the products of which are now considered to be transaminases involved in biosynthesis of sugar portions of cell-surface polysaccharides and aminoglycosides. The porR mutant showed a pleiotropic phenotype: delayed maturation of fimbrillin, preferential presence of Rgp and Kgp proteinases in culture supernatants, and no haemagglutination. The porR mutant had altered phenol extractable polysaccharide compared to the porR(+) sibling strain. A mAb, 1B5, that reacts with sugar portions of P. gingivalis cell surface polysaccharide and membrane-type Rgp proteinase showed no reaction with the cell lysates of the porR mutant. These results indicate that porR is involved in biosynthesis of cell surface polysaccharide that may function as an anchorage for Rgp, Kgp, haemagglutinins and the haemoglobin receptor protein.

References

Apr 15, 1979·The Biochemical Journal·H N ShahR A Williams
Sep 1, 1988·Journal of Medical Microbiology·A S McKeeP D Marsh
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Jan 1, 1994·Annual Review of Microbiology·H W Liu, J S Thorson
Nov 15, 1994·FEMS Microbiology Letters·C I Hoover, F Yoshimura
Jun 1, 1994·Periodontology 2000·A D Haffajee, S S Socransky
Aug 28, 1998·Microbiology·Ben N FryBernard A M van der Zeijst
Apr 15, 2000·Microbial Pathogenesis·T ChenM J Duncan
Jan 12, 2001·Oral Microbiology and Immunology·S I FarquharsonG R Gray

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Citations

Jun 2, 2015·Nihon saikingaku zasshi. Japanese journal of bacteriology·Mikio ShojiMiki Kawada-Matsuo
Nov 26, 2003·Microbiology and Immunology·Yoshiaki HasegawaFuminobu Yoshimura
Oct 13, 2015·Microbial Pathogenesis·Mikio Shoji, Koji Nakayama
Jun 21, 2007·Microbiology and Immunology·Keitarou Saiki, Kiyoshi Konishi
Nov 9, 2012·Molecular Oral Microbiology·D OsbourneH M Fletcher
Jan 3, 2013·Molecular Oral Microbiology·A W AruniH M Fletcher
Dec 19, 2009·Periodontology 2000·Rishi D PathiranaEric C Reynolds
Apr 10, 2010·Microbiology·Devon O OsbourneHansel M Fletcher
Jan 18, 2005·FEMS Microbiology Reviews·Teresa OlczakCaroline Attardo Genco
Apr 26, 2012·FEMS Microbiology Letters·Nagihan Bostanci, Georgios N Belibasakis
Jun 8, 2012·International Journal of Evolutionary Biology·Ponnerassery S SudheeshSaoud Al-Habsi
Mar 1, 2012·Journal of Oral Microbiology·Christina O IgboinEugene J Leys
Jan 21, 2017·Molecular Oral Microbiology·M RangarajanM A Curtis
Jan 5, 2018·Journal of Dental Research·G GabarriniA J van Winkelhoff
Nov 8, 2017·Nihon saikingaku zasshi. Japanese journal of bacteriology·Koji Nakayama
Jun 27, 2007·Nature Biotechnology·Eric DuchaudAbdenour Benmansour
Apr 12, 2017·Journal of Bacteriology·Surashree S KulkarniMark J McBride
Sep 24, 2013·Journal of Bacteriology·Minnie RangarajanMichael A Curtis
Sep 15, 2012·Nihon saikingaku zasshi. Japanese journal of bacteriology·Mikio Shoji
Sep 5, 2008·Current Protocols in Microbiology·Myriam BélangerAnn Progulske-Fox
Feb 26, 2005·Infection and Immunity·Elaine VanterpoolHansel M Fletcher

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