PMID: 2124547Nov 26, 1990Paper

Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/beta-galactosidase/galactokinase

FEBS Letters
P LjungcrantzK Mosbach

Abstract

The in-frame gene fusion between 3 enzymes, galactose dehydrogenase, beta-galactosidase and galactokinase, is described. The purified artificial tripartite enzyme displayed all three enzymic activities. Two major forms of the hybrid protein were found, consisting of 4 and 8 subunits respectively, but other forms could also be identified. Each subunit was made up of one monomer each of galactose dehydrogenase, beta-galactosidase and galactokinase. Proximity effects exhibited by the hybrid enzyme could be demonstrated using [14C]galactose as a reporter molecule.

References

Jan 1, 1976·Annual Review of Biochemistry·K Kirschner, H Bisswanger
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis

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Citations

Dec 19, 2006·Plant Biotechnology Journal·Lauralynn KourtzKristi D Snell
Mar 16, 2021·Frontiers in Microbiology·Mengyu ZhouYingjin Yuan
Dec 1, 1993·Microbiological Reviews·M Riley
Aug 30, 2008·Current Opinion in Biotechnology·Robert J ConradoMatthew P DeLisa

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