Construction and characterization of ack knock-out mutants of Propionibacterium acidipropionici for enhanced propionic acid fermentation

Biotechnology and Bioengineering
Supaporn SuwannakhamShang-Tian Yang

Abstract

Propionibacterium acidipropionici produces propionic acid from glucose with acetic acid, succinic acid, and CO2 as byproducts. In this work, inactivation of ack gene, encoding acetate kinase (AK), by gene disruption and integrational mutagenesis was studied as a method to reduce acetate formation in propionic acid fermentation. The partial ack gene of approximately 750 bp in P. acidipropionici was cloned using a PCR-based method with degenerate primers and sequenced. The deduced amino acid sequence had 88% similarity and 76% identity with the amino acid sequence of AK from Bacillus subtilis. The partial ack gene was used to construct a linear DNA fragment with an inserted tetracycline resistance cassette and a nonreplicative integrational plasmid containing a tetracycline resistance gene cassette. These DNA constructs were then introduced into P. acidipropionici by electroporation, resulting in two mutants, ACK-Tet and TAT-ACK-Tet, respectively. Southern hybridization confirmed that the ack gene in the mutant ACK-Tet was disrupted by the inserted tetracycline resistance gene. As compared to the wild-type, the activities of AK were reduced by 26% and 43% in ACK-Tet and TAT-ACK-Tet mutants, respectively. The specific growth rate ...Continue Reading

References

Oct 1, 1982·Journal of Bacteriology·C S Harwood, E Canale-Parola
Nov 1, 1993·Journal of Bacteriology·F J GrundyT M Henkin
Jul 15, 1993·Biochemical and Biophysical Research Communications·E C Chan
May 1, 1993·Journal of Bacteriology·W R McClearyA J Ninfa
Dec 29, 1995·Gene·A M Guérout-FleuryP Stragier
Apr 7, 1999·Biotechnology and Bioengineering·E M Green, G N Bennett
Feb 7, 2001·Applied and Environmental Microbiology·J P JoreP H Pouwels
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Aug 3, 2004·Science·Holger BrüggemannGerhard Gottschalk
Oct 20, 2005·Journal of Bioscience and Bioengineering·Pornpimon Kiatpapan, Yoshikatsu Murooka
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yongzhe PiaoYoshikatsu Murooka
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yongzhe PiaoYoshikatsu Murooka
Dec 20, 1991·Biotechnology and Bioengineering·J C Diaz-RicciJ E Bailey
Sep 1, 1991·Biotechnology and Bioengineering·S T Hsu, S T Yang
May 1, 1994·Biotechnology and Bioengineering·S T YangG Hong

❮ Previous
Next ❯

Citations

Apr 12, 2013·Applied Microbiology and Biotechnology·Ehab Mohamed AmmarShang-Tian Yang
Jul 19, 2012·World Journal of Microbiology & Biotechnology· PoonamRameshwar Singh
Aug 31, 2013·Chemical Reviews·Adrie J J Straathof
Mar 15, 2014·Journal of Industrial Microbiology & Biotechnology·Chris C StowersBrandon A Rodriguez
Jun 2, 2015·Journal of Food Science and Technology·Hamidreza HajfarajollahAli Afaghi
Dec 17, 2014·Journal of Industrial Microbiology & Biotechnology·Chandresh ThakkerGeorge N Bennett
May 31, 2011·International Journal of Food Microbiology·Anne ThierryGwenaël Jan
Jun 17, 2009·Biotechnology and Bioengineering·An Zhang, Shang-Tian Yang
Oct 21, 2016·Biotechnology Journal·Carlos H Luna-FloresEsteban Marcellin
Nov 24, 2017·Applied Microbiology and Biotechnology·Kamil PiwowarekIwona Ścibisz
Feb 6, 2017·Biotechnology Letters·Ismail EşMohamed Koubaa
May 2, 2021·Applied and Environmental Microbiology·Bo ZhangTimothy J Hackmann
Aug 20, 2021·Applied Microbiology and Biotechnology·Ehab M Ammar, George P Philippidis
Sep 21, 2010·Journal of Microbiological Methods·Meike SörensenHolger Brüggemann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.