Construction and characterization of an experimental ISCOMS-based hepatitis B polypeptide vaccine

World Journal of Gastroenterology : WJG
Xiao-Ju GuanYan Tang

Abstract

To characterize the biochemical and immunological properties of an experimental ISCOMS vaccine prepared from a novel therapeutic polypeptide based on T cell epitopes of HBsAg, and a heptatis B-ISCOMS was prepared and investigated. An immunostimulating complexes(ISCOMS)-based vaccine containing a novel therapeutic hepatitis B polypeptide was prepared by dialysis method, and its formation was visualized by electron microscopy and biochemically verified by SDS-polyacrylamide gel electrophoresis. Amount of the peptide within ISCOMS was determined by Bradford assay, and specific CTL response was detected by ELISPOT assay. Typical cage-like structures of submicroparticle with a diameter of about 40nm were observed by electron microscopy. Results from Bradford assay showed that the level of peptide incorporation was about 0.33g.L(-1). At the paralleled position close to the sixth band of the molecular weight marker(3480kDa) a clear band was shown in SDS-PAGE analysis, indicating successful incorporation of polypeptide into ISCOMS. It is suggested that ISCOMS delivery system could efficiently improve the immunogenicity of polypeptide and elicit specific immune responses in vivo by the results of ELISPOT assay, which showed that IFN-gam...Continue Reading

References

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Jan 31, 2002·World Journal of Gastroenterology : WJG·Hui LiShu-Sheng Wang
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Citations

Mar 13, 2003·World Journal of Gastroenterology : WJG·Hong ChengFu-Cheng Ma
Jul 11, 2003·World Journal of Gastroenterology : WJG·Chuan-Lin DingJiang-Ying Xu
Mar 8, 2005·Immunology and Cell Biology·Megan T SandersMartin J Pearse
Feb 5, 2004·World Journal of Gastroenterology : WJG·Chang-Zheng SongZeng-Liang Bai
Jan 1, 2003·World Journal of Gastroenterology : WJG·De-Wei DuYong-Ying Zhou
May 24, 2011·International Journal of Pharmaceutics·Asa SchiöttNikolai Kirkby

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