Construction and characterization of an infectious cDNA clone of coxsackievirus A 10

Virology Journal
Qiliang LiuHongbo Liu

Abstract

Coxsackievirus A10 (CA10) constitutes one of the four major pathogens causing hand, foot and mouth disease in infants. Infectious clones are of great importance for studying viral gene functions and pathogenic mechanism. However, there is no report on the construction of CA10 infectious clones. The whole genome of CA10 derived from a clinical isolate was amplified into two fragments and ligated into a linearized plasmid vector in one step by In-Fusion Cloning. The obtained CA10 cDNA clones and plasmids encoding T7 RNA polymerase were co-transfected into 293 T cells to rescue CA10 virus. The rescued virus was identified by SDS-PAGE, Western blotting and transmission electron microscopic. One-day-old ICR mice were intracerebrally inoculated with the CA10 virus and clinical symptoms were observed. Multiple tissues of moribund mice were harvested for analysis of pathogenic changes and viral distribution by using H&E staining, real-time PCR and immunohistochemical staining. CA10 viruses were rescued from the constructed cDNA clone and reached a maximum titer of 108.125TCID50/mL after one generation in RD cells. The virus exhibited similar physical and chemical properties to those of the parental virus. It also showed high virulence ...Continue Reading

References

Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R Kandolf, P H Hofschneider
Jan 14, 2004·The Journal of General Virology·Yi-Chun ChenHuan-Yao Lei
Apr 16, 2005·The Journal of General Virology·Minetaro AritaTatsuo Miyamura
May 23, 2007·Journal of Medical Virology·A PaananenM Roivainen
Mar 2, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Soile BlomqvistMerja Roivainen
Dec 17, 2011·Emerging Infectious Diseases·Maria A BrachoAntonio Salazar
Jan 3, 2012·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Meghan E FuschinoKirsten St George
Mar 13, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A MirandH Peigue-Lafeuille
Sep 7, 2012·Journal of Virology·Qunying MaoJunzhi Wang
Dec 24, 2013·Journal of Virological Methods·Natallia V LazouskayaPeter A Barton
May 26, 2015·Virus Research·Wangheng HouNingshao Xia
Jul 14, 2016·Medical Microbiology and Immunology·S AswathyrajD Hober
Jun 20, 2017·Antiviral Research·Shuxuan LiNingshao Xia
Sep 16, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ashok MunivenkatappaDevendra T Mourya
Dec 15, 2018·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Minh Tu Van HoangH Rogier van Doorn

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transmission electron microscopy
electron microscopy

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.