Construction and characterization of genetically-marked bivalent anti-Shigella dysenteriae 1 and anti-Shigella flexneri Y live vaccine candidates

Microbial Pathogenesis
S R KleeC A Guzman

Abstract

Bivalent vaccine candidates were developed against Shigella dysenteriae 1 and Shigella flexneri, which are among the most frequent causative agents of shigellosis in developing countries. The rfp and rfb gene clusters, which code for S. dysenteriae serotype 1 O-antigen biosynthesis, were inserted into an arsenite resistance minitransposon and randomly integrated into the attenuated S. flexneri aroD serotype Y strain SFL124. Nine recombinant clones that efficiently expressed both homologous and heterologous O-antigens were obtained. Southern blot analysis showed that in one clone the S. dysenteriae 1 genes had integrated into the chromosome, whereas in all the others they had integrated into the virulence plasmid. All recombinant clones exhibited normal growth characteristics, were able to invade and survive within eukaryotic cells to the same extent as the parental strain, and expressed efficiently the recombinant lipopolysaccharide within invaded cells. Immunization of mice with two of the recombinant clones resulted in the production of antibodies specific for both homologous and heterologous O-antigens. The recombinant clones constitute promising vaccine candidates which can readily be distinguished from endemic shigellae by...Continue Reading

References

Mar 1, 1991·Reviews of Infectious Diseases·A A LindbergA Weintraub
Jun 1, 1991·Microbiological Reviews·T L Hale
Nov 1, 1991·Infection and Immunity·A B HartmanK H Eckels
Aug 1, 1990·Molecular & General Genetics : MGG·D O'Callaghan, A Charbit
Jun 1, 1990·Microbial Pathogenesis·A A LindbergB A Stocker
Jun 1, 1989·The Journal of Infectious Diseases·H L DuPontS B Formal
Jul 1, 1987·Microbial Pathogenesis·P J Sansonetti, J Mounier
Jun 1, 1967·Proceedings of the Society for Experimental Biology and Medicine·S B FormalE H LaBrec
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
May 1, 1982·The Medical Clinics of North America·M M Levine
Mar 1, 1982·Infection and Immunity·P J SansonettiS B Formal
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S M HallingN Kleckner
Feb 1, 1984·The Journal of Infectious Diseases·E C TramontS B Formal
Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Dec 1, 1993·Infection and Immunity·M B Goldberg, P J Sansonetti

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Citations

Oct 7, 2011·Beilstein Journal of Organic Chemistry·Abhishek Santra, Anup Kumar Misra
Feb 21, 2004·FEMS Microbiology Reviews·Amy V Jennison, Naresh K Verma
Jan 10, 2014·Clinical and Vaccine Immunology : CVI·R W KaminskiE V Oaks
Jun 30, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·G DouganC Simmons
Feb 27, 2001·Biomolecular Engineering·B Drabner, C A Guzmán
May 20, 2008·International Journal of Medical Microbiology : IJMM·Gábor NagyTibor Pál

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