Construction and characterization of single-gene chromosomes in Saccharomyces cerevisiae

Journal of Bioscience and Bioengineering
Kazuo YamagishiSatoshi Harashima

Abstract

Based on a previously developed PCR-mediated chromosome splitting method, a genome engineering technique was developed in haploid Saccharomyces cerevisiae for incorporating any desired chromosomal region into a chromosome that carries a single gene. Based on the viability of cells carrying an essential gene in such a construct, close physical proximity of two telomeres and a centromere does not appear to compromise gene function. Spontaneous loss of constructed single-gene chromosomes during vegetative growth was high (0.2-0.4 per cell division), suggesting the possibility of creating novel cells carrying single-gene chromosomes derived from various chromosomal regions in a variety of combinations by exploiting combinatorial loss.

References

Apr 25, 1990·Nucleic Acids Research·C Sheehan, A S Weiss
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Oct 6, 2001·Science·M K RaghuramanW L Fangman
Oct 29, 2003·The Journal of Cell Biology·Stefan WestermannGeorjana Barnes
May 3, 2005·Applied Microbiology and Biotechnology·Yeonhee KimSatoshi Harashima
Jul 16, 2005·BioTechniques·Minetaka SugiyamaSatoshi Harashima
Oct 20, 2005·Journal of Bioscience and Bioengineering·Donny WidiantoSatoshi Harashima
Mar 1, 2006·Applied Microbiology and Biotechnology·Minetaka SugiyamaSatoshi Harashima
Jun 2, 2006·Nucleic Acids Research·Yeon-Hee KimSatoshi Harashima
Jul 19, 2006·Genes & Development·Conrad A NieduszynskiAnne D Donaldson
Mar 9, 2007·Applied Microbiology and Biotechnology·Kiriko MurakamiMasafumi Nishizawa

❮ Previous
Next ❯

Citations

Mar 3, 2012·Journal of Bioscience and Bioengineering·Youji UedaSatoshi Harashima
Aug 18, 2009·Applied Microbiology and Biotechnology·Minetaka SugiyamaSatoshi Harashima

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.