PMID: 9537754Apr 16, 1998Paper

Construction and characterization of type 1 non-fimbriate and non-adhesive mutants of Salmonella typhimurium

FEMS Immunology and Medical Microbiology
L S HancoxSteven Clegg

Abstract

Mutations in the fimH gene of Salmonella typhimurium result in a non-fimbriate, non-adhesive phenotype. This phenotype was shown to be due to the lack of both fimH and fimF expression since disruption of the fimH gene by insertion of a DNA cassette into this determinant results in mutants that are complemented by plasmids carrying both fimH and fimF. Deletion mutations within the S. typhimurium fimH gene carried on a recombinant plasmid can be used to complement the mutant, and these transformants are non-adhesive but fully fimbriate, consistent with the role of FimH as being necessary for fimbrial adhesin expression. Adherence to erythrocytes, HeLa, and Hep-2 cells is associated with expression of the FimH polypeptide, and fimbriate strains that cannot synthesize FimH are non-adhesive. Discrete differences in the amino acid sequences of the adhesive type 1 and the non-hemagglutinating type 2 FimH polypeptides were detected, and are most likely responsible for the differences in hemagglutinating activity.

References

Jun 1, 1991·Microbial Pathogenesis·D L SwensonD C Old
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S J HultgrenS Normark
Aug 1, 1970·Journal of Bacteriology·D C Old, J P Duguid
Nov 4, 1969·Journal of Medical Microbiology·J P Duguid, I Campbell
Nov 1, 1980·Journal of Bacteriology·T K KorhonenN Kuusi
Aug 1, 1983·Journal of Medical Microbiology·A TavendaleJ P Duguid
Dec 1, 1995·Journal of Bacteriology·K S YehS Clegg

❮ Previous
Next ❯

Citations

Jul 29, 2011·The Journal of Biological Chemistry·Dagmara I KisielaEvgeni V Sokurenko
Jan 25, 2002·Journal of Bacteriology·Steven Clegg, Kelly T Hughes
Jun 12, 2012·PLoS Pathogens·Dagmara I KisielaEvgeni V Sokurenko
Aug 24, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·D KisielaM Ugorski
Jan 24, 2008·The Journal of Biological Chemistry·Daniela ChessaAndreas J Baümler
Aug 5, 2017·Frontiers in Cellular and Infection Microbiology·Krzysztof GrzymajloPeter Schierack
Jun 13, 2020·Molecular Biology Reports·Zeynep EranNefise Akçelik
Feb 20, 1999·The Journal of Biological Chemistry·K ThankavelS N Abraham
Jun 18, 2020·International Journal of Molecular Sciences·Beata KlasaKrzysztof Grzymajło
May 30, 2019·Frontiers in Microbiology·Rafal KolendaKrzysztof Grzymajlo
Sep 16, 2019·Microbial Pathogenesis·Tayyab RehmanPing Ouyang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.