Construction and diversity analysis of a murine IgE phage surface display library

Cell Research
Z D Li, M Yeh

Abstract

To make further investigation of the IgE antibody repertoire in Trichosanthin (TCS) allergic responses, a murine IgE phage surface display library was constructed (3.0 x 10(5) independent clones). We first constructed the V epsilon cDNA library (4.6 x 10(5) independent clones) and V kappa cDNA library (3.0 x 10(5) independent clones). Then, the V epsilon and V kappa gene segments were amplified from both libraries by PCR respectively, and assembled into Fab fragment by SOE PCR. The phage library containing Fabs was thus constructed. The diversity of V epsilon from this library was analyzed and proved. Fab clones with high specificity to TCS have been screened out.

References

Jan 1, 1991·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S H SparholtC Schou
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R OrlandiG Winter

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