PMID: 12884619Jul 30, 2003Paper

Construction and expression of the recombinant plasmid pcDNA3.1/Ts87 of Trichinella spiralis

Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
Ya-Ping YangPascal Boireau

Abstract

To construct and express DNA plasmid of Trichinella spiralis. The BamHI and HindIII enzyme sites and KOZAK sequences were introduced at both ends of Ts87 gene by PCR. Ts87 gene was ligated into pcDNA3.1(+) vector with T4 ligase. The recombinant plasmid pcDNA3.1/Ts87 and plasmid pcDNA3.1 were transfected into an eukaryotic cell line COS7 through Lipofectamine, respectively. The BALB/c mice were immunized with the purified plasmid DNA pcDNA3.1/Ts87 through two routes: intramuscular injection and gene-gun injection. The pcDNA3.1/Ts87 was expressed both in COS7 and in the BALB/c mice.

Related Concepts

Cercopithecus tantalus
Mice, Inbred BALB C
Episomes
Transfection
Trichinella spiralis
DNA, Helminth
Polynucleotide Vaccines
COS-7 Cells

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