Construction and functional analysis of fatty acid desaturase gene disruptants in Candida albicans

Microbiology
Somay Yamagata MurayamaSusumu Kajiwara

Abstract

Polyunsaturated fatty acids (PUFAs), including linoleic acid (C18 : 2) and alpha-linolenic acid (C18 : 3), are major components of membranes. PUFAs are produced from monounsaturated fatty acids by several fatty acid desaturases (FADs) in many fungi, but Saccharomyces cerevisiae, Schizosaccharomyces pombe and humans do not have these enzymes. Although the fungal pathogen Candida albicans produces C18 : 2 and C18 : 3, the enzymes that synthesize them have not yet been investigated. In this report, two ORFs, CaFAD2 and CaFAD3, were identified based on their homology to other yeast FADs, and CaFAD2 and CaFAD3 gene disruptants were constructed. Cafad2Delta and Cafad3Delta lost their ability to produce C18 : 2 and C18 : 3, respectively. Furthermore, S. cerevisiae cells expressing CaFad2p converted palmitoleic acid (C16 : 1) and C18 : 1 to hexadecadienoic acid (C16 : 2) and C18 : 2, respectively, and CaFad3p-expressing cells converted C18 : 2 to C18 : 3. These results strongly supported that CaFAD2 encodes the Delta12 FAD and that CaFAD3 encodes the omega3 FAD. However, phenotypic analysis demonstrated that the presence of these PUFAs did not affect the virulence to mice, or morphogenesis in the culture media used to induce morphologi...Continue Reading

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Citations

Mar 10, 2009·Eukaryotic Cell·Katharina TrunkMartine Raymond
Jul 21, 2006·Journal of Molecular Biology·Eleonora R SetiadiJoachim F Ernst
Jan 11, 2014·Biochimica Et Biophysica Acta·Mariam AyyashPeter Oelkers
Jul 26, 2017·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Michal TupecIva Pichová
Sep 24, 2013·Biological Research for Nursing·Toshiko OgawaShinobu Okada
Apr 9, 2017·World Journal of Microbiology & Biotechnology·Rosa SantomartinoMichele M Bianchi

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