Construction and immunogenicity of recombinant fowlpox vaccines coexpressing HA of AIV H5N1 and chicken IL18

Vaccine
Ma MingxiaoZhang Yingjiu

Abstract

cDNAs of the HA genes of subtype H5N1 AIV were fused to form a single open reading frame, designated H5HA-H7HA. The H5HA-H7HA cDNA and chicken Interleukin-18 (IL18) were inserted into the fowlpox virus (FPV) expression vector pUTA-16-LacZ to produce pUTAL-H5-H7-IL18. cDNA of H5N1 AIV HA was inserted into the FPV expression vector pUTA2 to create the recombinant expression plasmid pUTA2-H5. Plasmids were then co-transected into CEF cells. The two recombinant fowlpox viruses (rFPV) were produced by three cycles with the BrdU and verified by RT-PCR, IFA and Western blotting. One-day-old specific pathogen free (SPF) chickens and 7-day-old commercial Leghorn egg-laying chickens were inoculated with 10(6) PFU recombinant or parental fowlpox vaccine viruses by wing-web puncture. Hemagglutination inhibition (HI) antibody titer and nonspecific cellular immunity level were assessed after 1-3 weeks post-immunization. We found that all rFPV-vaccinated groups produced HI-specific antibodies, and the level of cellular immunity induced by the rFPV-H5-H7-IL18 strain was significantly higher than that induced by rFPV-H5HA. At 3 weeks post-inoculation, immunized SPF and Leghorn chickens were challenged with H5N1 HP AIV. The rFPV-H5-H7-IL18 vacci...Continue Reading

References

Dec 1, 1988·Vaccine·J Taylor, E Paoletti
May 10, 1986·The Veterinary Record·D J Alexander, R E Gough
Jul 31, 1998·Vaccine·E C ClaasA D Osterhaus
Oct 29, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K SchneiderK C Weining
Jan 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ron A M FouchierAlbert D M E Osterhaus

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Citations

Jan 16, 2014·Epidemiology and Infection·E M AbdelwhabT C Mettenleiter
Feb 9, 2008·Emerging Infectious Diseases·Azizul HaqueLloyd H Kasper
Oct 13, 2011·Expert Review of Vaccines·Erin L LousbergJohn D Hayball
Apr 9, 2015·Viruses·Lucas Sánchez-SampedroMariano Esteban
Jun 23, 2009·Veterinary Immunology and Immunopathology·Rong-Lan YinNai-Sheng Zhang
Nov 18, 2014·Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere·E F Kaleta
Oct 23, 2012·Vaccine·Md Masudur Rahman, Seong Kug Eo
Dec 23, 2014·Virus Research·Shouwen DuNingyi Jin
Sep 8, 2009·FEMS Immunology and Medical Microbiology·Hong-Ying ChenShi-Min Li
Jul 28, 2016·Human Vaccines & Immunotherapeutics·Rory D de Vries, Guus F Rimmelzwaan

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