Construction and immunogenicity prediction of Plasmodium falciparum CTL epitope minigene vaccine

Science in China. Series C, Life Sciences
Y Tang, H Wang

Abstract

The minigenes encoding Plasmodium falciparum CTL epitopes restricted to human MHC class I molecular HLA-A2 and HLA-B51, which were both at high frequency among Chinese population, were constructed as mono-epitope CTL vaccines named pcDNA3.1/tr and pcDNA3.1/sh. The minigenes of the two epitopes were then tandem linked to form a dimeric CTL epitope minigene recombinant vaccine. After DNA transfection, the epitope minigenes were expressed respectively in two human cell lines, each bearing one MHC class I molecule named CIR/HLA-A2.1 and K562/HLA-B51. The intracellular expression of the CTL epitope minigenes not only enhanced the stability of HLA-A2.1 and HLA-B51 molecules but also increased the assemblage of MHC class I molecules on cell surfaces, which testified the specific process and presentation of those endogenous expressed epitopes. For the cells transfected with the dimeric minigene encoding two tandem linked epitopes, the expression and presentation of each epitope were also detected on cell membranes that bore different MHC class I molecules. It meant that the adjacency of the two CTL epitopes did not interfere with the specific process and presentation of each epitope. Compared with the ordinary CTL studies that inoculat...Continue Reading

References

Jul 1, 1995·Journal of Leukocyte Biology·M E PerkusE Paoletti
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·H ShenJ F Miller
Mar 1, 1995·Parasitology Today·G J Waine, D P McManus

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Citations

Sep 27, 2014·International Journal of Cosmetic Science·C Stiefel, W Schwack

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