Construction and potential application of controlled autolytic systems for Lactobacillus casei in cheese manufacture

Journal of Food Protection
Yi Xu, Jian Kong


The rapid release of intracellular enzymes into the curd by the autolysis of lactic acid bacteria starters is universally recognized as a critical biological process to accelerate cheese ripening. Lactobacillus casei is typically the dominant nonstarter lactic acid bacterium in the ripening cheese. In this study, two controlled autolytic systems were established in L. casei BL23, based on the exploitation of the autolysins sourced from Lactococcus lactis (AcmA) and Enterococcus faecalis (AtlA). The lysis abilities of the systems were demonstrated both in broth and a model cheese, in which a fivefold increase in lactate dehydrogenase activity was detected in the curd with sufficient viable starter cells being maintained, indicating that they could lead to the timely release of intracellular enzymes.


Jan 1, 1991·Methods in Enzymology·D HanahanF R Bloom
Oct 23, 1997·Nature Biotechnology·P G de RuyterW M de Vos
Dec 16, 2003·International Journal of Food Microbiology·C L RandazzoC Caggia
May 8, 2004·Letters in Applied Microbiology·R Cibik, M-P Chapot-Chartier
Jun 15, 2004·International Journal of Food Microbiology·Valérie GagnaireJoëlle Léonil
Oct 17, 2006·Journal of Bacteriology·Catherine EckertStéphane Mesnage
Apr 10, 2007·Microbial Cell Factories·Stéphane HazebrouckPhilippe Langella
Mar 17, 2010·Applied and Environmental Microbiology·Régis StentzClaire Shearman


Dec 9, 2017·Frontiers in Microbiology·Wenyi ZhangZhihong Sun
Mar 9, 2021·Frontiers in Microbiology·Learn-Han LeeNurul-Syakima Ab Mutalib

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