Construction and preliminary immunobiological characterization of a novel, non-reverting, intranasal live attenuated whooping cough vaccine candidate

Journal of Microbiology and Biotechnology
Renee Cornford-NairnsTrilochan K S Mukkur

Abstract

We describe the construction and immunobiological properties of a novel whooping cough vaccine candidate, in which the aroQ gene, encoding 3-dehydroquinase, was deleted by insertional inactivation using the kanamycin resistance gene cassette and allelic exchange using a Bordetella suicide vector. The aroQ B. pertussis mutant required supplementation of media to grow but failed to grow on an unsupplemented medium. The aroQ B. pertussis mutant was undetectable in the trachea and lungs of mice at days 6 and 12 post-infection, respectively. Antigen-specific antibody isotypes IgG1 and IgG2a, were produced, and cell-mediated immunity [CMI], using interleukin-2 and interferon-gamma as indirect indicators, was induced in mice vaccinated with the aroQ B. pertussis vaccine candidate, which were substantially enhanced upon second exposure to virulent B. pertussis. Interleukin- 12 was also produced in the aroQ B. pertussis-vaccinated mice. On the other hand, neither IgG2a nor CMI-indicator cytokines were produced in DTaP-vaccinated mice, although the CMI-indicator cytokines became detectable post-challenge with virulent B. pertussis. Intranasal immunization with one dose of the aroQ B. pertussis mutant protected vaccinated mice against an ...Continue Reading

Citations

Mar 28, 2013·BMC Infectious Diseases·Elena ChiappiniMaurizio de Martino
Aug 3, 2014·Expert Review of Vaccines·Camille Locht, Nathalie Mielcarek
Oct 19, 2017·Australian and New Zealand Journal of Public Health·Tom Kovitwanichkanont
Nov 23, 2017·Medical Microbiology and Immunology·Dorji DorjiTrilochan K Mukkur
Jan 30, 2019·Frontiers in Immunology·Luis Solans, Camille Locht
Apr 4, 2021·Vaccines·Camille Locht
Jul 3, 2021·Frontiers in Immunology·Violaine Dubois, Camille Locht

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