PMID: 15367355Sep 16, 2004Paper

Construction and screening of the human phage antibody library from a patient with melanoma

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Jun YangChun-ying Li

Abstract

To construct and screen the human phage antibody library from a patient with melanoma. Total RNA was extracted from the peripheral blood mononuclear cells (PBMCs) of a patient with melanoma who has survived for twenty years. The heavy chain Fd fragments and kappa light chains were amplified by RT-PCR. The amplified products were cloned into phagemid vector to construct Fab antibody library. The library was panned with two kinds of melanoma cells for 4 rounds. Antibody library contained 1.2 x 10(8) different clones. Specific phage antibodies against one of the two melanoma cells were obtained after 4 rounds of panning. Specific human phage antibodies against melanoma cells were obtained.

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