Construction and use of a zebrafish heart voltage and calcium optical mapping system, with integrated electrocardiogram and programmable electrical stimulation

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Eric LinGlen F Tibbits

Abstract

Zebrafish are increasingly being used as a model of vertebrate cardiology due to mammalian-like cardiac properties in many respects. The size and fecundity of zebrafish make them suitable for large-scale genetic and pharmacological screening. In larger mammalian hearts, optical mapping is often used to investigate the interplay between voltage and calcium dynamics and to investigate their respective roles in arrhythmogenesis. This report outlines the construction of an optical mapping system for use with zebrafish hearts, using the voltage-sensitive dye RH 237 and the calcium indicator dye Rhod-2 using two industrial-level CCD cameras. With the use of economical cameras and a common 532-nm diode laser for excitation, the rate dependence of voltage and calcium dynamics within the atrial and ventricular compartments can be simultaneously determined. At 140 beats/min, the atrial action potential duration was 36 ms and the transient duration was 53 ms. With the use of a programmable electrical stimulator, a shallow rate dependence of 3 and 4 ms per 100 beats/min was observed, respectively. In the ventricle the action potential duration was 109 ms and the transient duration was 124 ms, with a steeper rate dependence of 12 and 16 ms ...Continue Reading

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Citations

Jun 28, 2016·American Journal of Physiology. Heart and Circulatory Physiology·Matthew R StoyekFrank M Smith
Jan 26, 2018·Journal of Cardiovascular Development and Disease·Silja BurkhardJeroen Bakkers
Oct 31, 2019·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christina M HullTom W Claydon
Oct 13, 2020·Frontiers in Physiology·Maedeh Bazmi, Ariel L Escobar
Jan 29, 2021·Journal of Cardiovascular Development and Disease·Celine F SantiagoDiane Fatkin
Dec 18, 2020·Frontiers in Physiology·Yonghe DingXiaolei Xu
Jul 2, 2021·Methods : a Companion to Methods in Enzymology·Alia ArslanovaGlen F Tibbits

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