PMID: 9647815Jul 2, 1998Paper

Construction and use of an ipb DNA module to generate Pseudomonas strains with constitutive trichloroethene and isopropylbenzene oxidation activity

Applied and Environmental Microbiology
F BerendesG Gottschalk

Abstract

Pseudomonas sp. strain JR1 exhibits trichloroethene (TCE) oxidation activity with isopropylbenzene (IPB) as the inducer substrate. We previously reported the genes encoding the first three enzymes of the IPB-degradative pathway (ipbA1, ipbA2, ipbA3, ipbA4, ipbB, and ipbC) and identified the initial IPB dioxygenase (IpbA1 A2A3A4) as responsible for TCE cooxidation (U. Pflugmacher, B. Averhoff, and G. Gottschalk, Appl. Environ. Microbiol. 62:3967-3977, 1996). Primer extension analyses revealed multiple transcriptional start points located upstream of the translational initiation codon of ipbA1. The transcription from these start sites was found to be IPB dependent. Thirty-one base pairs upstream of the first transcriptional start point tandemly repeated DNA sequences overlapping the -35 region of a putative sigma 70 promoter were found. These repeats exhibit significant sequence similarity to the operator-promoter region of the xyl meta operon in Pseudomonas putida, which is required for the binding of XylS, a regulatory protein of the XylS (also called AraC) family. These similarities suggest that the transcription of the IPB dioxygenase genes is modulated by a regulatory protein of the XylS/AraC family. The construction of an i...Continue Reading

References

Dec 1, 1992·Applied and Environmental Microbiology·M S Shields, M J Reagin
Jul 1, 1991·Applied and Environmental Microbiology·M S ShieldsP H Pritchard
Jan 1, 1990·Archives of Microbiology·J EwersH J Knackmuss
May 1, 1990·Applied and Environmental Microbiology·B R FolsomP H Pritchard
Nov 1, 1989·Applied and Environmental Microbiology·L P WackettR S Hanson
Mar 15, 1989·Biochemical and Biophysical Research Communications·D ArcieroA B Hooper
Jul 1, 1988·Applied and Environmental Microbiology·L P Wackett, D T Gibson
Oct 14, 1970·Journal of Molecular Biology·M Mandel, A Higa
Nov 1, 1966·The Biochemical Journal·R C BaylyD T Gibson
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Apr 1, 1994·Journal of Bacteriology·K FurukawaK Nakamura
Oct 1, 1989·Applied and Environmental Microbiology·L P Wackett, S R Householder

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