Construction of 1 mm microdialysis probe for amino acids dialysis in rats

Journal of Neuroscience Methods
W M RennoA J Beitz

Abstract

We describe here a microdialysis probe with 1 mm opening for precise and confined dialysis area in the awake, freely moving rat. This probe is designed to allow the local diffusion of the perfusion medium to an area approximately 175 microm high, 266 microm wide (mediolateral direction), and 305 microm in rostrocaudal direction. In addition, the probe allows the local application of drugs to the same precise area of interest. The probe was constructed from a piece of 25 gauge tubing with 1 mm hallowed opening located 0.5 mm from the distal (inserting) end. The dialysis fiber which was inserted into the stainless steel 25 gauge tubing and cemented into place has 200 microm diameter and 5000 molecular weight cut off. We tested the probe diffusion extent by direct infusion of fluorogold through the dialysis cannula. Changes in the extracellular concentrations of amino acids were measured in response to infusion of veratridine a sodium channel activator. All amino acids tested showed a significant 80% times decrease in their recovery concentration when compared to their respective concentrations recovered through 2 mm probe constructed earlier in our laboratory (Renno et al., 1992). Tests in awake rats with probes in the ventrocaud...Continue Reading

References

Jan 1, 1992·Journal of Neuroscience Methods·S R SkillingA A Larson
Jul 1, 1992·Journal of Neuroscience Methods·E P Frothingham, A I Basbaum
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Dec 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J Beitz
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Nov 1, 1991·Journal of Neuroscience Methods·T J BazzettR L Albin
Mar 1, 1990·Trends in Pharmacological Sciences·G Di Chiara
Jan 1, 1990·Progress in Neurobiology·H Benveniste, P C Hüttemeier
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R M Caprioli, S N Lin
Oct 17, 1989·European Journal of Pharmacology·J GarthwaiteS Moncada
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Dec 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M Y GlobusM D Ginsberg
Jun 1, 1989·Journal of Neurochemistry·H Benveniste
Jan 1, 1989·Progress in Neurobiology·R J Huxtable
Apr 1, 1989·Brain Research Bulletin·N Quan, C M Blatteis
Sep 1, 1986·Acta Physiologica Scandinavica·U Tossman, U Ungerstedt
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G Di Chiara, A Imperato
Aug 15, 1988·Neuroscience Letters·J M SolísR Martín del Río
Jan 1, 1984·Acta Psychiatrica Scandinavica. Supplementum·M Herrera-MarschitzU Ungerstedt
Apr 16, 1982·Neuroscience Letters·T ZetterströmB B Fredholm
Apr 1, 1993·Journal of Neuroscience Methods·T P ObrenovitchL Symon

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