Construction of 700 human/mouse A9 monochromosomal hybrids and analysis of imprinted genes on human chromosome 6

Journal of Human Genetics
J InoueMitsuo Oshimura

Abstract

As an in vitro assay system for the identification of human imprinted genes, a library of human/mouse A9 monochromosomal hybrids containing a single, intact bsr-tagged human chromosome of known parental origin, derived from normal human fibroblasts, has been previously generated by microcell-mediated chromosome transfer (MMCT). To supplement this assay system, we constructed additional 700 A9 monochromosomal hybrids, using a pSTneo or pPGKneo selection marker. To validate the A9 hybrids, we screened them with chromosome-specific polymorphic markers, and identified the hybrids containing either human chromosome 6, 7, 14, 18, or 21 of known parental origin. Matching paternal and maternal chromosome pairs of A9 hybrids were identified for chromosomes 6, 7, 14, and 18. The paternal-specific expression of ZAC (zinc finger protein, which regulates apoptosis and cell cycle arrest) and HYMAI (hydatidiform mole-associated and imprinted transcript), and the maternal-specific methylation of a CpG island within an imprinted domain on human chromosome 6q24, were maintained in A9 hybrids. For an example, we profiled the expression of expressed sequence tags (ESTs) and the methylation of CpG islands in the 300-kb imprinted domain around 6q24,...Continue Reading

Citations

Nov 24, 2012·Cell Division·Natalia CanelDaniel Salamone
Feb 7, 2008·Reproductive Biomedicine Online·Mitsuo Oshimura, Motonobu Katoh
Jun 8, 2011·Birth Defects Research. Part A, Clinical and Molecular Teratology·Jorge A Piedrahita
Feb 7, 2002·Genomics·Kazuhiko NakabayashiStephen W Scherer
Dec 7, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Amy M BremanBrenda R Grimes
Dec 12, 2017·Briefings in Functional Genomics·Shriram N Rajpathak, Deepti D Deobagkar
Dec 4, 2002·Genes, Chromosomes & Cancer·Hiroyuki KugohMitsuo Oshimura
May 2, 2003·Annals of the New York Academy of Sciences·Yinhua YuRobert C Bast

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