Construction of a 1.5-Mb bacterial artificial chromosome contig in Xp11.23, a region of high gene content

Genomics
K M BoycottN T Bech-Hansen

Abstract

To generate sequence-ready templates for the gene-rich Xp11.23 region, we have constructed a 1.5-Mb bacterial artificial chromosome (BAC) contig spanning the interval between the DNA markers OATL1 and DXS255. The contig includes 28 BACs, ranging in size from 58 to 258 kb with an average size of 135 kb, which provide 2.5-fold coverage of the region. The BAC contig was constructed based entirely on the content of 40 DNA markers from a previously established YAC contig and 11 new markers developed from BAC-end DNA sequences, 4 of which were required to close gaps in the map. There was no evidence of rearrangement, instability, or chimerism in any of the BAC clones. The BAC cloning system appears to provide robust and total physical coverage of this gene-rich region with clones that are suitable for DNA sequencing.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H ShizuyaM Simon
Mar 11, 1992·Nucleic Acids Research·U J KimM I Simon
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C PierceN Sternberg
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N Sternberg
Jan 1, 1997·Cytogenetics and Cell Genetics·K M BoycottN T Bech-Hansen

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