PMID: 8963899Mar 1, 1996Paper

Construction of a 750-kb bacterial clone contig and restriction map in the region of human chromosome 21 containing the progressive myoclonus epilepsy gene

Genome Research
N E StoneR M Myers

Abstract

The gene responsible for progressive myoclonus epilepsy of the Unverricht-Lundborg type (EPM1) is located on human chromosome 21q22.3 in a region defined by recombination breakpoints and linkage disequilibrium. As part of an effort to clone the EPM1 gene on the basis of its chromosomal location, we have constructed a 753-kb bacterial clone contig that encompasses the region containing the gene. Because DNA markers from the region did not identify intact yeast artificial chromosome (YAC) clones after screening several libraries, we built the contig from cosmid clones and used bacterial artificial chromosome (BAC) and bacteriophage P1 clones to fill gaps. In addition to constructing the clone contig, we determined the locations of the EcoRI, SacII, EagI, and NotI restriction sites in the clones, resulting in a high-resolution restriction map of the region. Most of the contig is represented by a level of redundancy that allows the orders of most restriction sites to be determined, provides multiple data points supporting the clone orders and orientations, and allows a set of clones with a minimum degree of overlap to be chosen for efficient additional analysis. The clone and restriction maps are in excellent agreement with maps ge...Continue Reading

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A E LehesjokiA de la Chapelle
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·N S ShepherdN Sternberg
Oct 1, 1993·Journal of Medical Genetics·A de la Chapelle
Aug 1, 1993·Nature Genetics·H IchikawaM Ohki

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Citations

Nov 25, 1997·Gene·R G LafrenièreG A Rouleau
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·A de la Chapelle, F A Wright
Aug 14, 1999·Epilepsia·A E Lehesjoki, M Koskiniemi
Apr 28, 2012·Neurobiology of Disease·Andrea BuzziMichele Simonato
Nov 22, 2007·Epilepsia·Tarja JoensuuOuti Kopra
Jul 2, 2015·ACS Chemical Neuroscience·Cornelia HainerNatalia Alenina
Aug 7, 1998·Genomics·S E Antonarakis
Jun 1, 1997·American Journal of Human Genetics·M Xiong, S W Guo
Oct 2, 2001·American Journal of Medical Genetics·A V Delgado-EscuetaK Yamakawa
Oct 28, 1997·American Journal of Medical Genetics·I N BespalovaM Burmeister
Jun 7, 2020·Applied and Environmental Microbiology·Pei-Ying HongChangzhi Wang

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