Abstract
In this study, an artificial multi-functional extracellular matrix (ECM) protein, tethered with a growth factor, was developed for neurite outgrowth induction. The designed ECM protein was comprised of an elastin-like peptide, as a structural unit, as well as the AG73 peptide sequence derived from the laminin and the C3 peptide sequence, which binds to neural cell adhesion molecules (derived from a synthetic peptide library) as functional units. Both AG73 and C3 have been demonstrated to promote cell adhesion and enhance neurite outgrowth. For the tethering of basic fibroblast growth factor (bFGF) to the ECM protein, helical peptides were fused to the ECM protein to form a coiled-coil helical structure with helical peptide-fused bFGF. Neurite outgrowth was induced in the PC12 cells that were cultured on this ECM protein as a result of the tethered-bFGF. Moreover, neurite outgrowth was enhanced by the AG73 and C3 peptides of the ECM protein.
References
Dec 1, 1992·Journal of Cellular Physiology·D S GrantH K Kleinman
Jul 31, 1989·Biochemical and Biophysical Research Communications·G C SephelH K Kleinman
Oct 15, 1988·Gene·G L Hu, K E Neet
Jan 27, 1995·Cell·C J Marshall
Oct 10, 1996·Experimental Cell Research·B L RichardH K Kleinman
Feb 1, 1996·Protein Expression and Purification·D T McPhersonD W Urry
Sep 23, 1998·Experimental Cell Research·B S WeeksH K Kleinman
Jul 20, 1999·The Journal of Biological Chemistry·E ChevetM D Katinka
Oct 3, 1999·Nature Biotechnology·L C RønnJ L Saffells
Jul 19, 2000·Journal of Neurochemistry·L C RønnE Bock
Sep 12, 2000·Nature Cell Biology·I Ivankovic-DikicI Dikic
Jun 26, 2001·Clinical and Experimental Pharmacology & Physiology·M Ford-PerrissM Murphy
Nov 17, 2001·Biomacromolecules·E KobatakeM Aizawa
Oct 1, 1993·Current Biology : CB·E K O'SheaP S Kim
Feb 25, 2006·Biomaterials·Imen ElloumiEiry Kobatake
Feb 9, 2007·Archives of Biochemistry and Biophysics·Mayumi MochizukiMotoyoshi Nomizu
Nov 27, 2007·Nature Biotechnology·Gabsang LeeLorenz Studer
Apr 22, 2008·Biomaterials·Makiko NakamuraEiry Kobatake
Sep 16, 2008·Cellular and Molecular Life Sciences : CMLS·S M HansenE Bock
Jun 3, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Makiko NakamuraEiry Kobatake
Jun 26, 2010·Journal of Cellular Physiology·Chan-Young JeonJae-Bong Park
Apr 26, 2011·Biomaterials·Aijun WangSong Li
May 5, 2011·Stem Cell Research & Therapy·Stephanie M Willerth
Aug 9, 2011·Bioconjugate Chemistry·Eiry KobatakeMasayasu Mie
Feb 8, 2013·Biomaterials·Yasmine AssalEiry Kobatake
Citations
Jun 9, 2016·Bioconjugate Chemistry·Nihad AdnanEiry Kobatake
Mar 14, 2020·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Afroza SharminEiry Kobatake
Apr 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·SokeLee SiewEiry Kobatake
Jul 14, 2018·Neurochemical Research·Chengyan ChuShen Li
Jun 17, 2015·Bioconjugate Chemistry·Yasmine AssalEiry Kobatake