Construction of a biocompatible decellularized porcine hepatic lobe for liver bioengineering

The International Journal of Artificial Organs
Kamal H HusseinHeung M Woo

Abstract

One of the major obstacles in applying decellularized organs for clinical use is the recellularization step, during which huge numbers of cells are required to develop whole livers. We established a simple protocol for constructing a bioartificial hepatic lobe and investigated its biocompatibility. The right lateral lobe of porcine liver was decellularized using 0.1% sodium dodecyl sulfate through the right branch of the portal vein. Decellularized lobes were evaluated by histological and biochemical analyses. DNA content was quantified to validate the decellularization protocol. The presence of immunogenic and pathogenic antigens was checked to exclude potential rejection and thrombosis after xenotransplantation. Xeno-reactivity of decellularized tissue against human peripheral blood mononuclear cells was examined. Cytotoxicity was evaluated against hepatocarcinoma cells. Finally, scaffolds were incubated in collagenase for biodegradation testing. The decellularized lobe preserved the three-dimensional architecture, ultrastructure, extracellular matrix components, and vasculature. Scaffolds were almost depleted of DNA in addition to antigenic and pathogenic antigens, which are considered barriers to xenotransplantation. The hu...Continue Reading

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Dec 18, 2013·The International Journal of Artificial Organs·Kamal H HusseinHeung M Woo

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Citations

Aug 16, 2016·The International Journal of Artificial Organs·Andrea Remuzzi
Dec 30, 2016·Journal of Anatomy·Khan L JunatasKirsti Witter
Mar 24, 2018·Journal of Biomedical Materials Research. Part a·Kamal Hany HusseinHeung-Myong Woo

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Methods Mentioned

BETA
biopsy
Protein Assay
PCR
electrophoresis
xenograft

Software Mentioned

SPSS
Image J

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