Construction of a eukaryotic expression vector pEGFP-C1-BMP-2 and its effect on cell migration.

Journal of Zhejiang University. Science. B
Xiao-ying WangYue-min Ding

Abstract

Bone morphogenetic proteins (BMPs) are known to play an important role in bone and cartilage development. Recent research has shown that BMPs may induce tumorigenesis and promote tumor to spread, but the molecular mechanisms have not been elucidated. The aim of the present study was to investigate the regulatory function of BMP-2 in the migration of COS-7 cells and the underlying mechanisms. Human BMP-2 genetic fragment was amplified and introduced into the pEGFP-C1 vector. After being confirmed by XhoI and BamHI digestion analyses and DNA sequencing, the recombinant pEGFP-C1-BMP-2 plasmid was transfected into COS-7 cells. The influence of BMP-2 on cell migration and cofilin activity was detected by cell scratch assay and Western blotting. The recombinant pEGFP-C1-BMP-2 was effectively expressed in COS-7 cells. An increased phosphorylation of both LIMK1 and cofilin and an enhancement of cell migration were observed in cells with overexpression of BMP-2. A recombinant pEGFP-C1-BMP-2 vector was successfully constructed and overexpression of BMP-2 regulated the activities of the downstream molecules of the Rho GTPase signaling pathway, which might contribute to the enhancement of cell migration.

References

Nov 12, 1965·Science·M R Urist
Nov 21, 1998·Nature·G von Heijne
Oct 25, 2002·Journal of Cellular Biochemistry·Jörg FiedlerRolf E Brenner
Sep 10, 2003·The Journal of Cell Biology·Victoria C FolettaJuliana Soosairaiah
May 21, 2005·Mechanisms of Development·Allan M GoldsteinDrucilla J Roberts
Aug 23, 2008·Journal of Cellular Physiology·Yi-Chin FongChih-Hsin Tang
Mar 19, 2009·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·M Blanco CalvoE Grande Pulido
Apr 14, 2009·Developmental Biology·Jennifer C HockingSarah McFarlane
Nov 10, 2009·Cytokine & Growth Factor Reviews·Christina SieberPetra Knaus
Jan 1, 2010·Neurosurgery·Jayesh P ThawaniPaul Park
Aug 7, 2010·Cytokine & Growth Factor Reviews·Ashok Singh, Rebecca J Morris
Feb 8, 2011·PloS One·Cristina GamellFrancesc Ventura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.