Construction of a genetically engineered microorganism for phenanthrene biodegradation

Journal of Basic Microbiology
Ying ZhouDongzhi Wei

Abstract

The bacterium Pseudomonas sp. CGMCC2953, isolated from oil-polluted soil, was used as a recipient for a biodegradative gene encoding catechol 2,3-dioxygenase (C23O), which was successfully cloned into the plasmid pK4 derived from pRK415 with a broad host range. The apparent phenanthrene biodegradation parameters of the recombinant microorganism (Pseudomonas sp. CGMCC2953-pK) were determined and compared with those of the wild type. As the key enzyme of phenanthrene degradation, C23O, could be expressed constitutively in the recombinant strain, Pseudomonas sp. CGMCC2953-pK showed an increased ability to degrade phenanthrene. The excessive production of C23O in Pseudomonas sp. CGMCC2953-pK could serve as an effective approach to construct genetically engineered microorganisms for the bioremediation of environmental contaminations.

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Citations

Jun 9, 2020·Archives of Microbiology· Sakshi, A K Haritash
Jul 25, 2021·The Science of the Total Environment·Manuel Méndez García, Martha Patricia García de Llasera
May 8, 2018·Environmental Science & Technology·Longfei JiangGan Zhang

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