PMID: 8995706Feb 1, 1997Paper

Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts

Journal of Virology
Ute GeigenmüllerS M Matsui

Abstract

We have constructed a genome-length cDNA clone for human astrovirus serotype 1. When a human colon cancer-derived cell line, CaCo-2, is transfected with RNA transcribed in vitro from this cDNA clone, infectious virus is produced at titers close to those observed after infection with intact astrovirus. A rodent cell line, BHK, which is largely refractory to astrovirus infection, was found to support efficient growth of the virus if transfected with viral RNA. The high transfection efficiency seen in the BHK cells allows studies of the viral replication in the transfected cells and thus should prove useful for the characterization of noninfectious astroviral mutants.

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Citations

Jun 21, 2002·The Journal of General Virology·Ute GeigenmüllerSuzanne M Matsui
Dec 26, 2001·The Journal of General Virology·David Kiang, Suzanne M Matsui
Apr 4, 2015·PloS One·Susana GuixRosa M Pintó
Oct 4, 2014·Clinical Microbiology Reviews·Albert BoschSusana Guix
Jan 21, 2017·Viruses·Carlos F Arias, Rebecca M DuBois
Jul 14, 2017·Journal of Virology·Andrew B JanowskiDavid Wang
Jan 19, 2002·Journal of Virology·Ute GeigenmüllerSuzanne M Matsui
Aug 15, 2020·Nature Communications·Valeria Lulla, Andrew E Firth
Sep 7, 2019·Virology Journal·Ruihua ZhangShijin Jiang
Feb 12, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ulrich Desselberger
Feb 17, 2018·Archives of Virology·Yifeng QinWeijian Huang
Jul 20, 2020·Current Opinion in Virology·Carlos Sandoval-Jaime
Jul 3, 2021·Viruses·Nicole Wildi, Torsten Seuberlich

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