Construction of a high-density genetic map and mapping of QTLs for soybean (Glycine max) agronomic and seed quality traits by specific length amplified fragment sequencing

BMC Genomics
Yanwei ZhangRan Xu

Abstract

Soybean is not only an important oil crop, but also an important source of edible protein and industrial raw material. Yield-traits and quality-traits are increasingly attracting the attention of breeders. Therefore, fine mapping the QTLs associated with yield-traits and quality-traits of soybean would be helpful for soybean breeders. In the present study, a high-density linkage map was constructed to identify the QTLs for the yield-traits and quality-traits, using specific length amplified fragment sequencing (SLAF-seq). SLAF-seq was performed to screen SLAF markers with 149 F8:11 individuals from a cross between a semi wild soybean, 'Huapidou', and a cultivated soybean, 'Qihuang26', which generated 400.91 M paired-end reads. In total, 53,132 polymorphic SLAF markers were obtained. The genetic linkage map was constructed by 5111 SLAF markers with segregation type of aa×bb. The final map, containing 20 linkage groups (LGs), was 2909.46 cM in length with an average distance of 0.57 cM between adjacent markers. The average coverage for each SLAF marker on the map was 81.26-fold in the male parent, 45.79-fold in the female parent, and 19.84-fold average in each F8:11 individual. According to the high-density map, 35 QTLs for plant...Continue Reading

References

May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·K G LarkJ H Orf
Mar 14, 2002·Plant Physiology·Dörte KlausPeter Dörmann
May 14, 2003·The EMBO Journal·Changcheng XuChristoph Benning
Apr 7, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·W-K ZhangS-Y Chen
Jun 29, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D L HytenM E Schmidt
Nov 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hans van OsHerman J van Eck
Jan 16, 2010·Nature·Jeremy SchmutzScott A Jackson
Aug 7, 2010·BMC Plant Biology·Andrew J SeverinRandy C Shoemaker
Apr 7, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yingpeng HanWenbin Li
Oct 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S H LeeH R Boerma
Nov 1, 1996·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M A MianH R Boerma
Mar 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B W DiersR C Shoemaker
Apr 11, 2014·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shin KatoMasao Ishimoto
Sep 25, 2016·Proceedings of the National Academy of Sciences of the United States of America·Dayong LiLihuang Zhu
Apr 9, 2017·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bin LiJunming Sun

❮ Previous
Next ❯

Citations

Jan 22, 2021·Journal of Bioinformatics and Computational Biology·Md Jahangir AlamMd Nurul Haque Mollah

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
electrophoresis
genotyping
RNA-seq

Software Mentioned

SLAF
Multiple array viewer
ICIMapping
Excel
seq
SPSS
Glyma
ICIM
MSTmap
SMOOTH

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.