Construction of a High-Density Genetic Map and Analysis of Seed-Related Traits Using Specific Length Amplified Fragment Sequencing for Cucurbita maxima

Frontiers in Plant Science
Yunli WangShuping Qu

Abstract

Seed traits are agronomically important for Cucurbita breeding, but the genes controlling seed size, seed weight and seed number have not been mapped in Cucurbita maxima (C. maxima). In this study, 100 F2 individual derived from two parental lines, "2013-12" and "9-6", were applied to construct a 3,376.87-cM genetic map containing 20 linkage groups (LGs) with an average genetic distance of 0.47 cM using a total of 8,406 specific length amplified fragment (SLAF) markers in C. maxima. Ten quantitative trait loci (QTLs) of seed width (SW), seed length (SL) and hundred-seed weight (HSW) were identified using the composite interval mapping (CIM) method. The QTLs affecting SW, SL and HSW explained a maximum of 38.6%, 28.9% and 17.2% of the phenotypic variation and were detected in LG6, LG6 and LG17, respectively. To validate these results, an additional 150 F2 individuals were used for QTL mapping of SW and SL with cleaved amplified polymorphic sequence (CAPS) markers. We found that two major QTLs, SL6-1 and SW6-1, could be detected in both SLAF-seq and CAPS markers in an overlapped region. Based on gene annotation and non-synonymous single-nucleotide polymorphisms (SNPs) in the major SWand SL-associated regions, we found that two ge...Continue Reading

References

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Citations

Apr 9, 2020·Frontiers in Plant Science·Yu GuoFeishi Luan
Apr 4, 2021·International Journal of Molecular Sciences·Yuzi ShiYing Duan
May 2, 2021·Horticulture Research·Josué Barrera-RedondoLuis E Eguiarte

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Datasets Mentioned

BETA
PRJNA549786

Methods Mentioned

BETA
environmental stress
genotyping
PCR
electrophoresis
SLAF-seq

Software Mentioned

R package
SOAP
Primer Premier
ASMap
R
Excel
qtl package
SLAF
HighMap
Statistical Analysis System ( SAS )

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