Aug 23, 2012

Construction of a high-density genetic map for grape using next generation restriction-site associated DNA sequencing

BMC Plant Biology
Nian WangShaohua Li

Abstract

Genetic mapping and QTL detection are powerful methodologies in plant improvement and breeding. Construction of a high-density and high-quality genetic map would be of great benefit in the production of superior grapes to meet human demand. High throughput and low cost of the recently developed next generation sequencing (NGS) technology have resulted in its wide application in genome research. Sequencing restriction-site associated DNA (RAD) might be an efficient strategy to simplify genotyping. Combining NGS with RAD has proven to be powerful for single nucleotide polymorphism (SNP) marker development. An F1 population of 100 individual plants was developed. In-silico digestion-site prediction was used to select an appropriate restriction enzyme for construction of a RAD sequencing library. Next generation RAD sequencing was applied to genotype the F1 population and its parents. Applying a cluster strategy for SNP modulation, a total of 1,814 high-quality SNP markers were developed: 1,121 of these were mapped to the female genetic map, 759 to the male map, and 1,646 to the integrated map. A comparison of the genetic maps to the published Vitis vinifera genome revealed both conservation and variations. The applicability of nex...Continue Reading

  • References33
  • Citations42

References

Mentioned in this Paper

Grape extract
Chromosome Map
Genetic Markers
Vitis vinifera
Genome Mapping
Raisins
Sequencing
Massively-Parallel Sequencing
Chromosomes, Plant
Sequence Determinations, DNA

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