Construction of a high-density linkage map and mapping quantitative trait loci for somatic embryogenesis using leaf petioles as explants in upland cotton (Gossypium hirsutum L.)

Plant Cell Reports
Zhenzhen XuFuguang Li

Abstract

The first high-density linkage map was constructed to identify quantitative trait loci (QTLs) for somatic embryogenesis (SE) in cotton ( Gossypium hirsutum L.) using leaf petioles as explants. Cotton transformation is highly limited by only a few regenerable genotypes and the lack of understanding of the genetic and molecular basis of somatic embryogenesis (SE) in cotton (Gossypium hirsutum L.). To construct a more saturated linkage map and further identify quantitative trait loci (QTLs) for SE using leaf petioles as explants, a high embryogenesis frequency line (W10) from the commercial Chinese cotton cultivar CRI24 was crossed with TM-1, a genetic standard upland cotton with no embryogenesis frequency. The genetic map spanned 2300.41 cM in genetic distance and contained 411 polymorphic simple sequence repeat (SSR) loci. Of the 411 mapped loci, 25 were developed from unigenes identified for SE in our previous study. Six QTLs for SE were detected by composite interval mapping method, each explaining 6.88-37.07% of the phenotypic variance. Single marker analysis was also performed to verify the reliability of QTLs detection, and the SSR markers NAU3325 and DPL0209 were detected by the two methods. Further studies on the relative...Continue Reading

References

May 16, 2002·The Journal of Heredity·R E Voorrips
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J. ZhangT. Zhang
Feb 18, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·L F GaoJ Z Jia
Jan 5, 2005·Trends in Biotechnology·Rajeev K VarshneyMark E Sorrells
Aug 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Asuka NishimuraMakoto Matsuoka
Feb 28, 2006·Molecular Genetics and Genomics : MGG·James E FrelichowskiMauricio Ulloa
Nov 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ganesan SunilkumarKeerti S Rathore
Nov 7, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Huihui LiJiankang Wang
Oct 1, 1993·Genome Génome / Conseil National De Recherches Canada·K F Yu, K P Pauls
Nov 1, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Satya Narayan JenaSamir V Sawant
Aug 28, 2012·Nature Genetics·Kunbo WangShuxun Yu
Jun 1, 1987·Plant Cell Reports·N L Trolinder, J R Goodin
May 3, 2014·The New Phytologist·Zuoren YangFuguang Li
May 20, 2014·Nature Genetics·Fuguang LiShuxun Yu

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