Construction of a high-quality genomic BAC library for Chinese peanut cultivar Zhonghua 8 with high oil content

Botanical Studies
Yu-Ning ChenHui-Fang Jiang

Abstract

Arachis hypogaea L. (2n = 4× = 40, AABB) is one of the most important oil and economic crop plants in the word. This species has the largest genome size of about 2,813 Mb among the oil crop species. Zhonghua 8 is a peanut cultivar planted widely in central China and has several superior traits including high oil content, high yield and disease resistance. A high-quality BAC library of Zhonghua 8 was constructed for future researches on the genomics of Chinese peanut cultivars. A Hin d III-digested genomic BAC (bacterial artificial chromosome) library was constructed with the genomic DNA from leaves of Zhonghua 8. This BAC library consists of 160,512 clones and the average insert is estimated about 102 kb ranging from 30 to 150 kb. The library represents about 5.55× haploid genome equivalents, and provides a 99.71% probability of finding specific genes. The empty-vector rate is under 5 percent detected from 200 randomly selected clones. Probing of 384 clones with the psbA gene of barley chloroplast and the atp6 gene of rice mitochondrion indicated that the contamination with organellar DNA is insignificant. Successive subculture of three clones showed that the inserts are stable in one hundred generations. This study presented t...Continue Reading

References

Jul 29, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·B Yüksel, A H Paterson
Jan 31, 2008·BMC Plant Biology·Patricia M GuimarãesAngelique D'Hont
Dec 3, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·R K VarshneyD A Hoisington
Jun 8, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y P KhedikarR K Varshney
Dec 31, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·K RaviR K Varshney
Nov 11, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hongde QinBaozhu Guo
Nov 19, 2011·Biotechnology Advances·Manish K PandeyRajeev K Varshney

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Methods Mentioned

BETA
electrophoresis

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