Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis.

Applied and Environmental Microbiology
M van de GuchteG Venema

Abstract

A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 kilobase pairs in size, differ only in the type of antibiotic resistance they confer to their hosts. pMG36 carries a kanamycin resistance marker, which was replaced by an erythromycin resistance marker in pMG36e. As an example of the use of these vectors, the hen egg white lysozyme-coding sequence was inserted. A fusion protein of the expected size was detected in a transformed L. lactis subsp. lactis strain by using Western blotting (immunoblotting).

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Citations

Feb 1, 1992·FEMS Microbiology Reviews·M van de GuchteG Venema
Aug 5, 1999·Letters in Applied Microbiology·J K McCormickM E Stiles
May 23, 2002·FEMS Microbiology Letters·Mark Tangney, Gerald F Fitzgerald
Oct 26, 2002·Journal of Bacteriology·Julie D BouchardSylvain Moineau
Jan 9, 2004·Applied and Environmental Microbiology·Hikmate AbriouelCharles M A P Franz
Jun 20, 2007·Molecular Genetics and Genomics : MGG·Daniel StraumeDzung B Diep
Mar 11, 2009·Antimicrobial Agents and Chemotherapy·Zhizeng SunLiandong Huan
May 2, 2016·Journal of Dairy Science·Xiaoxuan GeJin Zhong
Apr 23, 2017·Applied and Environmental Microbiology·Narimane DahmaneSophie Payot
Oct 1, 1996·Antonie van Leeuwenhoek·M J Gasson
Aug 19, 2007·Journal of Bacteriology·Laetitia FontainePascal Hols
Jan 17, 2007·Applied Microbiology and Biotechnology·Xiaobo LiangLiandong Huan
Nov 13, 2010·Biotechnology Letters·Eun Ju Yang, Hae Choon Chang

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