PMID: 11313129Apr 21, 2001Paper

Construction of a modified mini-Tn5 luxCDABE transposon for the development of bacterial biosensors for ecotoxicity testing

FEMS Microbiology Letters
H J WeitzL A Glover

Abstract

A mini-Tn5 transposon was modified to introduce a promoterless luxCDABE cassette from Vibrio fischeri into environmentally relevant bacterial strains in order to develop bioluminescence-based biosensors for toxicity testing. The mini-Tn5 luxCDABE transposon was chromosomally integrated downstream from an active promoter into two Pseudomonas strains (Pseudomonas fluorescens 8866 and Pseudomonas putida F1). Characterisation of the bioluminescent transconjugants demonstrated that the transposon integration was stable and had no effect on growth rate. Both P. fluorescens 8866 Tn5 luxCDABE and P. putida F1 Tn5 luxCDABE were used to assess the toxicity of standard solutions (Cu, Zn and 3,5-DCP) as well as Cu- and 3,5-DCP-spiked groundwater samples. They were successfully used for bioluminescence-based bioassays and the potential value of using different bacterial biosensors for ecotoxicity testing was shown.

References

Jul 1, 1992·Journal of General Microbiology·G S Stewart, P Williams
Nov 1, 1990·Applied and Environmental Microbiology·E A RattrayL A Glover
Jul 10, 1981·Nucleic Acids Research·D Ish-Horowicz, J F Burke
May 1, 1995·Biotechnology Progress·B Volesky, Z R Holan
Jan 1, 1993·Advances in Microbial Physiology·E A Meighen, P V Dunlap
Aug 1, 1997·Applied and Environmental Microbiology·L D RasmussenT Barkay
Jan 22, 1998·Applied and Environmental Microbiology·M J HuertasJ L Ramos
May 26, 1999·FEMS Microbiology Letters·G M SinclairK Killham
Jun 22, 1999·Ecotoxicology and Environmental Safety·A C LaytonG S Sayler
Feb 1, 1957·Journal of Cellular Physiology·G COHEN-BAZIRER Y STANIER

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Citations

Oct 18, 2001·Current Opinion in Biotechnology·H P Schweizer
Jul 19, 2002·Environmental Microbiology·Hedda J WeitzKen Killham
Sep 27, 2002·Applied and Environmental Microbiology·C Y ShaoL A Glover
Jan 22, 2008·Analytica Chimica Acta·Stefano GirottiElisabetta Maiolini
Feb 24, 2009·Environmental Pollution·E E DiplockG I Paton
Feb 18, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Juan Manuel Bello-LópezJulieta Rojo-Medina
Jun 8, 2021·Biosensors & Bioelectronics·Michael MoraskieSylvia Daunert
Jun 19, 2021·Journal of Microbiological Methods·Jeffrey K Schachterle, Qi Huang
Aug 10, 2021·Frontiers in Plant Science·Jeffrey K Schachterle, Qi Huang
May 10, 2011·New Biotechnology·Pensri Plangklang, Alissara Reungsang

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