Construction of a nontoxigenic Clostridium botulinum strain for food challenge studies.

Applied and Environmental Microbiology
Marite BradshawEric A Johnson

Abstract

Clostridium botulinum produces the most poisonous natural toxin known and is a perennial concern to the food industry and to regulatory agencies due to the potential threat of food-borne botulism. To ensure the botulinal safety of foods, rigorous food challenge testing to validate food-processing conditions and food formulations has been routinely performed. Detection of the botulinum neurotoxin is performed by using a mouse bioassay and/or in vitro assays. There has been considerable interest by the food industry and regulatory agencies in minimizing or even replacing the use of animals in these challenge studies. In addition, due to stringent select-agent regulations, the testing of various foods using toxigenic C. botulinum strains requires facilities and personnel that are certified for work with this organism. For this purpose we propose to generate sets of nontoxigenic C. botulinum strains from proteolytic and nonproteolytic groups that differ from the wild-type strains only by their inability to produce botulinum neurotoxin. In this initial study we describe the generation of a nontoxigenic mutant of C. botulinum strain 62A using the ClosTron mutagenesis system by inserting a group II intron into the botulinum neurotoxin...Continue Reading

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Citations

May 1, 2012·Applied and Environmental Microbiology·David G KirkMiia Lindström
Mar 4, 2014·PloS One·Regina Clare Meyer WhitemarshSabine Pellett
Feb 25, 2014·Molecular Microbiology·Glen P CarterDena Lyras
Jan 17, 2017·Polish Journal of Veterinary Sciences·T GrendaK Kwiatek
Nov 10, 2011·Toxins·Andy Pickett, Karen Perrow

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Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

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Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.