Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL-18 delivered by Salmonella

Journal of Applied Microbiology
X GaoC Wang

Abstract

Genotype VII Newcastle disease (ND) is one of the most epidemic and serious infectious diseases in the poultry industry. A novel vaccine targeting VII Newcastle disease virus (NDV) is still proving elusive. In this study, we constructed regulated delayed lysis Salmonella strains expressing either a fusion protein (F) alone under an eukaryotic CMV promoter or together with chicken IL-18 (chIL-18) as a molecular adjuvant under prokaryotic Ptrc promoter, named pYL1 and pYL23 respectively. Oral immunization with recombinant strains induced NDV-specific serum IgG antibodies in both pYL1- and pYL23-immunized chickens. The presence of chIL-18 significantly increased lymphocyte proliferation in immunized chickens, as well as the percentages of CD3+ CD4+ and CD3+ CD8+ T cells in serum, even if a statistically significant difference did not exist. After a virulent challenge, pYL23 immunization provided about 80% protection at day 10 postinfection, compared with 60% of protection offered by pYL1 immunization and 100% protection in the inactivated vaccine group, indicating the enhanced immune response provided by chIL-18, which was also confirmed by histochemical analysis. Recombinant lysis Salmonella-vectored DNA vaccine could provide us ...Continue Reading

References

Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N L LeeR G Wallace
Sep 1, 2000·Advanced Drug Delivery Reviews·J Heppell, H L Davis
Oct 29, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·K SchneiderK C Weining
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ruxandra Draghia-AkliMarta L Fiorotto
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas W GöbelBernd Kaspers
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Wei KongRoy Curtiss
Apr 8, 2010·Critical Reviews in Immunology·Roy CurtissShifeng Wang
Jul 28, 2012·Avian Pathology : Journal of the W.V.P.A·Dennis J AlexanderChad M Fuller
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Wei KongRoy Curtiss
May 27, 2014·Research in Veterinary Science·Wenyan ShiXiaomin Zhao
Nov 24, 2017·Cytogenetic and Genome Research·Baifen SongYudong Cui
Dec 20, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Josephine E Clark-Curtiss, Roy Curtiss
Jul 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Weiwen DengThomas W Dubensky

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