Construction of a novel shuttle vector for use in Brevibacterium flavum, an industrial amino acid producer

Journal of Microbiological Methods
Daqing XuXiaoyuan Wang

Abstract

Brevibacterium flavum has been developed to produce amino acids l-valine, l-lysine and l-threonine. However, there are not enough vectors available for the research on metabolic engineering in Brevibacterium flavum. Here we have constructed a shuttle expression vector pDXW-8 between Escherichia coli and B. flavum. The vector harbors an origin oriE for replicating in E. coli, a second origin oriC for replicating in B. flavum, a large multiple cloning site including 11 single restriction enzyme sites and suitable for cloning multiple genes or large DNA fragments, a tac promoter and a lacI(PF104) fragment which tightly controls the tac promoter. The applicability of pDXW-8 was confirmed by the expression of the vhb gene in B. flavum. The vector pDXW-8 will be very useful for research on metabolic engineering in corynebacteria.

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Citations

Apr 27, 2011·Applied Microbiology and Biotechnology·Jan Nešvera, Miroslav Pátek
Jun 28, 2011·Journal of Industrial Microbiology & Biotechnology·Xiaohu HouWeiguo Zhang
Mar 26, 2013·Journal of Industrial Microbiology & Biotechnology·Chuanzhi ZhangJian Chen
Sep 14, 2013·Journal of Industrial Microbiology & Biotechnology·Jianzhong XuWeiguo Zhang
Apr 28, 2012·The Journal of Microbiology·Yang YangXiaoyuan Wang
Mar 15, 2015·Metabolic Engineering·Cheng ChenXiaoyuan Wang
Jul 29, 2015·Enzyme and Microbial Technology·Guoqiang HanXiaoyuan Wang
Feb 11, 2015·Journal of Industrial Microbiology & Biotechnology·Chuanzhi ZhangJian Chen
Jul 6, 2016·Journal of Industrial Microbiology & Biotechnology·Jianzhong XuWeiguo Zhang
Mar 30, 2018·Applied Microbiology and Biotechnology·Xiaoyuan WangPeter J Quinn
Feb 3, 2018·Applied Microbiology and Biotechnology·Natalya V GorshkovaSergey V Mashko
Oct 13, 2018·Biotechnology and Applied Biochemistry·Wenjian MaXiaoyuan Wang
May 6, 2019·Critical Reviews in Biotechnology·Ying-Yu WangWei-Guo Zhang
Mar 25, 2016·Biotechnology and Applied Biochemistry·Wenjian MaXiaoyuan Wang
Feb 13, 2017·Applied Microbiology and Biotechnology·Anqi ZhaoXiaoyuan Wang
Nov 18, 2018·Journal of Industrial Microbiology & Biotechnology·Milin ZhanYe Ni
Jan 22, 2019·Applied Microbiology and Biotechnology·Xiaoyuan Wang
Apr 27, 2021·Frontiers in Microbiology·Qingzhuo WangSheng Yang

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