Construction of a panel of canine-rodent hybrid cell lines for use in partitioning of the canine genome

Genomics
A A LangstonE A Ostrander

Abstract

We have constructed a collection of canine-rodent microcell hybrid cell lines by fusion of canine fibroblast microcell donors with immortalized rodent recipient cells. Characterization of the hybrid cell lines using a combination of fluorescence in situ hybridization and PCR analysis of canine microsatellite repeat sequences allowed selection of a panel of hybrids in which most canine chromosomes are represented. Approximately 90% of genetic markers and genes that were tested could be assigned to 1 of 31 anonymous canine chromosome groups, based on common patterns of retention in the hybrid set. Many of these putative chromosome groups have now been validated by linkage analysis. This panel of cell lines provides a tool for development of genetic, physical, and comparative maps of the canine genome.

References

Oct 1, 1975·Annals of Internal Medicine·R S Kucherlapati, F H Ruddle
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E A OstranderG Duyk
Jan 1, 1986·Cytogenetics and Cell Genetics·C Chevalet, F Corpet
Aug 1, 1986·Molecular and Cellular Biology·A D Miller, C Buttimore
Jan 1, 1966·Experimental Cell Research·J W Littlefield
Oct 1, 1971·The Biochemical Journal·S D Handmaker
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R E Fournier
Jun 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P WilliamsonY Boyd
Jan 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M Fredholm, A K Winterø
Mar 1, 1995·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E A OstranderJ Rine
Aug 1, 1993·Animal Genetics·N G HolmesJ Sampson
May 1, 1993·Genomics·H L DrwingaR A Mulivor
Apr 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·M L Guevara-FujitaG J Brewer
May 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L V FranciscoE A Ostrander
Feb 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·C F LangfordN P Carter
Jun 1, 1996·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·M SwitońskiP Fischer
Oct 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·L AnderssonJ Womack
Jan 1, 1996·Cytogenetics and Cell Genetics·A S DutraJ M Puck
Jan 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·P E FischerE P Nacheva
Feb 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A SabileP Avner
Mar 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·F LingaasG Dolf
Jan 27, 1998·Genomics·C S MellershE A Ostrander

❮ Previous
Next ❯

Citations

Aug 31, 2001·ILAR Journal·Matthew BinnsMatthew Breen
Apr 18, 1998·Proceedings of the National Academy of Sciences of the United States of America·G M AclandG D Aguirre
Nov 9, 2011·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Keiko MiyaderaGustavo D Aguirre
Jan 27, 1998·Genomics·C S MellershE A Ostrander
May 20, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R K Wayne, E A Ostrander
Nov 1, 2013·Oncology Reports·Gabriela B GelaletiDebora Ap P C Zuccari

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.