Construction of a Potential Breast Cancer-Related miRNA-mRNA Regulatory Network.

BioMed Research International
Xinhong LiuXin Zhao

Abstract

Breast cancer is a malignant tumor that occurs in the epithelial tissue of the breast gland and has become the most common malignancy in women. The regulation of the expression of related genes by microRNA (miRNA) plays an important role in breast cancer. We constructed a comprehensive breast cancer-miRNA-gene interaction map. Three miRNA microarray datasets (GSE26659, GSE45666, and GSE58210) were obtained from the GEO database. Then, the R software "LIMMA" package was used to identify differential expression analysis. Potential transcription factors and target genes of screened differentially expressed miRNAs (DE-miRNAs) were predicted. The BRCA GE-mRNA datasets (GSE109169 and GSE139038) were downloaded from the GEO database for identifying differentially expressed genes (DE-genes). Next, GO annotation and KEGG pathway enrichment analysis were conducted. A PPI network was then established, and hub genes were identified via Cytoscape software. The expression and prognostic roles of hub genes were further evaluated. We found 6 upregulated differentially expressed- (DE-) miRNAs and 18 downregulated DE-miRNAs by analyzing 3 Gene Expression Omnibus databases, and we predicted the upstream transcription factors and downstream target...Continue Reading

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Citations

Jul 3, 2021·Cells·Danyel Fernandes ContilianiTiago Campos Pereira

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Datasets Mentioned

BETA
GSE58210
GSE109169
GSE139038
and

Methods Mentioned

BETA
Transfection

Software Mentioned

Cytoscape ( cytoHubba )
miRNet
Gene Expression Profiling Interactive Analysis ( GEPIA )
Enrichr
Cytoscape
GraphPad Prism
GEPIA
R LIMMA
FunRich
cytoHubba

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