Construction of a Robust Sphingomonas sp. Strain for Welan Gum Production via the Expression of Global Transcriptional Regulator IrrE

Frontiers in Bioengineering and Biotechnology
Xiaoliu LiuSha Li

Abstract

Welan gum is a widely used microbial polysaccharide produced by Sphingomonas sp. However, an important factor hindering the expansion of its production is the maladaptation of strain to fermentation conditions. In this work, the global transcriptional regulator gene irre was selected as a stress-resistant element. And it was integrated into the site of the genomic carotene synthesis key enzyme gene crtB to construct a robust carotenoid-free welan gum producing strain. Fermentation with the recombinant strain effectively reduced the ethanol consumption and pigment content in the product. The tolerance temperature increased by 10°C without the need for controlling the pH. Under this fermentation condition, welan gum concentration could still reach 20.26 ± 0.25 g/L, which was 187.38% higher than that of the wild-type strain (7.05 ± 0.15 g/L). Transcriptome analysis showed that with the control of IrrE, more than 1000 genes that are involved in multiple pathways, including two-component system, bacterial chemotaxis, flagellar assembly, and cell cycle, exhibited changes at the transcriptional level and jointly allowed the strain to protect against environmental stresses.

References

Oct 1, 1992·Current Opinion in Genetics & Development·S Lindquist
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G A ArmstrongJ E Hearst
Jan 1, 1996·Advances in Biochemical Engineering/biotechnology·E A Johnson, W A Schroeder
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Jun 19, 2001·Trends in Biochemical Sciences·A H West, A M Stock
Oct 7, 2003·Annual Review of Microbiology·Tanja M Gruber, Carol A Gross
Dec 1, 2006·Nature·Emanuele G BiondiMichael T Laub
Feb 13, 2007·Metabolic Engineering·Hal Alper, Gregory Stephanopoulos
Feb 16, 2008·Current Opinion in Chemical Biology·Christine Nicole S Santos, Gregory Stephanopoulos
Oct 4, 2008·Genome Research·Haibao TangAndrew H Paterson
Jun 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jesse D Bloom, Frances H Arnold
Jul 20, 2010·Bioprocess and Biosystems Engineering·Hui LiPing-Kai Ouyang
Oct 10, 2012·Journal of Bacteriology·Xiaoyu WangPing Xu
Mar 12, 2013·Biotechnology Advances·Zhanglin LinJianqing Wang
Oct 30, 2013·Nature Reviews. Genetics·Jeffrey E Barrick, Richard E Lenski
May 31, 2015·Trends in Microbiology·Aindrila Mukhopadhyay
Dec 10, 2015·BioMed Research International·Anmin YuLi Liu
Apr 26, 2017·Journal of Industrial Microbiology & Biotechnology·Yukai SuoJufang Wang
Jul 5, 2017·Current Opinion in Biotechnology·Tong Un ChaeSang Yup Lee
Apr 8, 2018·Applied and Environmental Microbiology·Alexander B WestbyeJ Thomas Beatty
Nov 30, 2018·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Sharath NarayananSunish Kumar Radhakrishnan

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Citations

Dec 10, 2020·Biotechnology for Biofuels·Li WangYing-Jin Yuan
Feb 27, 2021·International Journal of Biological Macromolecules·Ming ZhaoHong Xu

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Datasets Mentioned

BETA
SRR11496385
SRR11496387

Methods Mentioned

BETA
gene modification
biosensor
electrophoresis
PCR
RNA-seq
phosphotransferase

Software Mentioned

BLAST

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