Construction of a subgenomic CV-B3 replicon expressing emerald green fluorescent protein to assess viral replication of a cardiotropic enterovirus strain in cultured human cells

Journal of Virological Methods
Michel WehbeAlexis Bouin

Abstract

Coxsackieviruses B (CV-B) (Picornaviridae) are a common infectious cause of acute myocarditis in children and young adults, a disease, which is a precursor to 10-20% of chronic myocarditis and dilated cardiomyopathy (DCM) cases. The mechanisms involved in the disease progression from acute to chronic myocarditis phase and toward the DCM clinical stage are not fully understood but are influenced by both viral and host factors. Subgenomic replicons of CV-B can be used to assess viral replication mechanisms in human cardiac cells and evaluate the effects of potential antiviral drugs on viral replication activities. Our objectives were to generate a reporter replicon from a cardiotropic prototype CV-B3/28 strain and to characterize its replication properties into human cardiac primary cells. To obtain this replicon, a cDNA plasmid containing the full CV-B3/28 genome flanked by a hammerhead ribozyme sequence and an MluI restriction site was generated and used as a platform for the insertion of sequences encoding emerald green fluorescent protein (EmGFP) in place of those encoding VP3. In vitro transcribed RNA from this plasmid was transfected into HeLa cells and human primary cardiac cells and was able to produce EmGFP and VP1-conta...Continue Reading

References

Apr 27, 2005·Journal of Molecular and Cellular Cardiology·Byung-Kwan LimEun-Seok Jeon
Nov 22, 2005·Nature Methods·Nathan C ShanerRoger Y Tsien
Aug 12, 2008·Virus Research·Olga Fernández-MiragallEncarnación Martínez-Salas
May 5, 2011·Journal of Biomedicine & Biotechnology·Alfredo CastellóLuis Carrasco
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Jul 4, 2012·Antimicrobial Agents and Chemotherapy·Jun ZuoPaul Krogstad
Jan 8, 2013·Future Virology·Amanda J Chase, Bert L Semler

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Citations

Jun 21, 2017·Journal of Virological Methods·Qing XiongYihong Peng

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