Construction of a trifunctional cellulase and expression in Saccharomyces cerevisiae using a fusion protein

BMC Biotechnology
Zi-Lu LiuZheng-Bing Jiang

Abstract

Cellulose is the most important component of lignocellulose, and its degradation requires three different types of enzymes to act synergistically. There have been reports of single gene duality, but no gene has been described to have more than two functions. Cloning and expression of fusion cellulases containing more than two kinds of catalytic domains has not been reported thus far. We synthesized three different cellulase genes and linked the three catalytic domains with a (G4S)3 flexible linker. The trifunctional cellulase gene (BCE) containing three types of cellulase functions was constructed and expressed in S. cerevisiae successfully. The β-glucosidase, the exoglucanase and the endoglucanase activity of the trifunctional cellulase BCE reached 16.80 IU/mg, 2.26 IU/mg and 20.67 IU/mg, which was 46.27, 6.73 and 46.20% higher than the activities of the β-glucosidase BG, the endoglucanase CBH and the endoglucanase EG. The filter paper enzyme activity of BCE was higher than those of BG, CBH and EG, reached 2.04 IU/mg. The trifunctional cellulase BCE was designed based on β-glucosidase BG, endoglucanase EG and exoglucanase CBH, and it possessed β-glucosidase activity, endoglucanase activity and exoglucanase activity simultaneou...Continue Reading

References

Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
May 13, 2006·Biotechnology Advances·Y-H Percival ZhangJonathan R Mielenz
Jun 9, 2009·Current Opinion in Biotechnology·David B Wilson
Dec 4, 2009·Applied Microbiology and Biotechnology·Alina Iulia ChiriacF I Javier Pastor
Jun 7, 2011·Applied and Environmental Microbiology·Nidhi AdlakhaSyed Shams Yazdani
Nov 3, 2012·The Journal of Biological Chemistry·Thomas GannerBernd Nidetzky
Apr 26, 2013·Applied Biochemistry and Biotechnology·Raja Tahir MahmoodSaqib Hussain Hadri
Feb 5, 2014·Bioresource Technology·Baljit KaurB S Chadha
Sep 12, 2014·Journal of Industrial Microbiology & Biotechnology·Wei WangDongzhi Wei
Jan 6, 2016·Critical Reviews in Food Science and Nutrition·Harsh P Sharma Sugandha
Apr 22, 2016·Analytical and Bioanalytical Chemistry·Alexandra GaubertArnaud Salvador
Jul 30, 2016·Applied Microbiology and Biotechnology·Steffi A DavisonWillem Heber van Zyl

❮ Previous
Next ❯

Citations

Feb 27, 2021·Frontiers in Bioengineering and Biotechnology·Hannah C YocumNancy A Da Silva

❮ Previous
Next ❯

Datasets Mentioned

BETA
HG313887.1
M19373
AJ133614
ABN52701.1
CAL48345
AGT15838
ADY18331

Methods Mentioned

BETA
PCR
electrophoresis
Protein Assay
Assay

Software Mentioned

SignalP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.