Construction of Agrobacterium strains by electroporation of genomic DNA and its utility in analysis of chromosomal virulence mutations.

Applied and Environmental Microbiology
T C CharlesE W Nester

Abstract

We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background but differ in their types of pTi (octopine or nopaline). Both nopaline and octopine pTi-carrying chvE mutants were deficient in vir regulon induction and exhibited similar reductions in host range.

References

Jan 1, 1991·Methods in Enzymology·G A CangelosiE W Nester
May 1, 1991·Plant Molecular Biology·T Mozo, P J Hooykaas
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G A CangelosiE W Nester
Oct 25, 1989·Nucleic Acids Research·W J Shen, B G Forde
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M D ChiltonE W Nester
Jan 1, 1993·Annual Review of Microbiology·B W Holloway
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D M RaineriE W Nester
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N ShimodaY Machida

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Citations

Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Luoping LiShen Q Pan
Jan 1, 1996·Critical Reviews in Biotechnology·S Y Ho, G S Mittal
Aug 28, 1999·Journal of Bacteriology·B W GoodnerG Grabowski

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