Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli

Journal of Industrial Microbiology & Biotechnology
Jin-Ying GuoXueli Zhang

Abstract

Glycerol, which is an inevitable by-product of biodiesel production, is an ideal carbon source for the production of carotenoids due to its low price, good availability and chemically reduced status, which results in a low requirement for additional reducing equivalents. In this study, an alternative carbon-utilization pathway was constructed in Escherichia coli to enable more efficient β-carotene production from glycerol. An aldehyde reductase gene (alrd) and an aldehyde dehydrogenase gene (aldH) from Ralstonia eutropha H16 were integrated into the E. coli chromosome to form a novel glycerol-utilization pathway. The β-carotene specific production value was increased by 50% after the introduction of alrd and aldH. It was found that the glycerol kinase gene (garK), alrd and aldH were the bottleneck of the alternative glycerol metabolic pathway, and modulation of garK gene with an mRS library further increased the β-carotene specific production value by 13%. Finally, co-modulation of genes in the introduced aldH-alrd operon led to 86% more of β-carotene specific production value than that of the strain without the alternative glycerol-utilization pathway and the glycerol-utilization rate was also increased. In this work, β-carote...Continue Reading

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Citations

Jun 26, 2020·Frontiers in Bioengineering and Biotechnology·Yuanqing WuXueming Zhao
Aug 28, 2020·Current Opinion in Biotechnology·Yuexuan ZuGregory Stephanopoulos
Aug 20, 2021·Metabolic Engineering·Jie WangXueli Zhang

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Methods Mentioned

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