Construction of broad-host-range vectors for general cloning and promoter selection in Pseudomonas and Escherichia coli

Gene
M A Farinha, A M Kropinski

Abstract

We have constructed two promoter-selection vectors based upon the broad-host-range plasmid pRO1614. pQF40 (6 kb) contains a promoterless tetA gene downstream from a large multiple cloning site while pQF26 (5.4 kb) possesses a promoterless cat cartridge. The latter vector displayed a copy number of 13 in Pseudomonas aeruginosa and 39 in Escherichia coli. When promoter sequences derived from the Pseudomonas phage phi PLS27 were cloned into pQF26, high levels of chloramphenicol-acetyltransferase were detected in P. aeruginosa. In E. coli the activity was approximately one-third that in P. aeruginosa when corrections were made for the plasmid copy number.

References

Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly

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Citations

Nov 15, 1995·Molecular & General Genetics : MGG·L SerinoD Haas
Jan 1, 1990·Biotechnology Advances·V N Iyer
Mar 1, 1990·Molecular Microbiology·D G StoreyB H Iglewski
Dec 13, 1997·Canadian Journal of Microbiology·B W McLeanA M Kropinski
Jul 17, 2003·Molecular Microbiology·Ulrich GerstelUte Römling
Sep 30, 2010·Evolution; International Journal of Organic Evolution·Tim Connallon, Andrew G Clark
May 18, 2013·Annals of the New York Academy of Sciences·Ward B Watt
Nov 10, 2020·Frontiers in Microbiology·Youssef El MoualiCarlos Balsalobre
Mar 1, 1995·Journal of Bacteriology·T L YahrD W Frank
Mar 1, 1992·Infection and Immunity·M J WickB H Iglewski

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