Construction of carotenoid biosynthetic pathways using squalene synthase

FEBS Letters
Maiko FurubayashiDaisuke Umeno

Abstract

The first committed steps of steroid/hopanoid pathways involve squalene synthase (SQS). Here, we report the Escherichia coli production of diaponeurosporene and diapolycopene, yellow C30 carotenoid pigments, by expressing human SQS and Staphylococcus aureus dehydrosqualene (C30 carotenoid) desaturase (CrtN). We suggest that the carotenoid pigments are synthesized mainly via the desaturation of squalene rather than the direct synthesis of dehydrosqualene through the non-reductive condensation of prenyl diphosphate precursors, indicating the possible existence of a "squalene route" and a "lycopersene route" for C30 and C40 carotenoids, respectively. Additionally, this finding yields a new method of colorimetric screening for the cellular activity of squalene synthases, which are major targets for cholesterol-lowering drugs.

References

Dec 14, 1978·Biochemical and Biophysical Research Communications·T NishinoH Katsuki
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K MathC D Poulter
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G A ArmstrongJ E Hearst
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·T NakashimaS Hata
Mar 21, 1998·Archives of Biochemistry and Biophysics·J F ThompsonH J Harwood
Jul 26, 2002·Journal of the American Chemical Society·Michael B JarstferC Dale Poulter
Jul 26, 2002·Journal of the American Chemical Society·Brian S J BlaggC Dale Poulter
Nov 12, 2002·Journal of Bacteriology·Daisuke UmenoFrances H Arnold
May 29, 2003·Chemistry & Biology·Pyung Cheon LeeClaudia Schmidt-Dannert
Jun 6, 2003·Applied and Environmental Microbiology·Daisuke Umeno, Frances H Arnold
Feb 20, 2004·Journal of Bacteriology·Daisuke Umeno, Frances H Arnold
Jun 4, 2005·Applied and Environmental Microbiology·Luan TaoQiong Cheng
Nov 5, 2005·Applied and Environmental Microbiology·Bosung KuJonathan S Dordick
Jan 11, 2007·Drugs·Valentine Charlton-Menys, Paul N Durrington
Apr 13, 2007·Archives of Biochemistry and Biophysics·Per Stickforth, Gerhard Sandmann
Sep 30, 2008·Applied and Environmental Microbiology·Pyung Cheon LeeClaudia Schmidt-Dannert
Oct 25, 2008·Archives of Biochemistry and Biophysics·Gerhard Sandmann

❮ Previous
Next ❯

Citations

Jan 19, 2016·Nature Chemical Biology·Feifei ChenLefu Lan
Aug 6, 2014·FEBS Letters·Maiko FurubayashiDaisuke Umeno
Oct 6, 2014·Journal of Bioscience and Bioengineering·Akinori KatabamiDaisuke Umeno
Oct 30, 2016·Protein Expression and Purification·Ming LiuJian-Qiang Kong
Mar 4, 2020·Molecular Biology and Evolution·Carlos Santana-MolinaDamien P Devos
Mar 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Micaela GianiRosa María Martínez-Espinosa
Apr 11, 2019·Frontiers in Bioengineering and Biotechnology·Nisarg GohilVijai Singh
Jul 29, 2018·Metabolic Engineering·Philip HartzFrank Hannemann
Jun 26, 2021·Scientific Reports·Claudine BaraquetCaroline S Harwood
Mar 27, 2020·Journal of Agricultural and Food Chemistry·Yafeng SongWim J Quax
Aug 14, 2021·Molecular Microbiology·Sandra RizkJames P Saenz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.