Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-γ and their efficacy against poultry coccidiosis

Research in Veterinary Science
Muhammad Ali A ShahXiangrui Li

Abstract

The study describes vaccination experiments with highly immunogenic sporozoite E. acervulina cSZ-2 co-administered with chicken IL-2 (chIL-2) and interferon-γ (chIFN-γ) to determine their efficacies against homologue challenge. The entire coding sequence of cSZ2, chIL-2 and chIFN-γ were cloned into eukaryotic expression vector pVAX1, constructing DNA vaccines pVAX1-cSZ2, pVAX1-chIL-2, pVAX1-chIFN-γ, pVAX1-cSZ2-chIL-2 and pVAX1-cSZ2-chIFN-γ. The expression of target genes in vivo was detected by RT-PCR and Western blot. Chicken experiments were carried out by vaccinating chickens two times at dose rate of 100 μg intramuscularly. At 28 days of age, all chickens were inoculated orally with 1×10(5) sporulated oocysts of E. acervulina except the unchallenged control group. Seven days after challenge, all chickens were weighted and slaughtered for duodenum collection. The results indicated that these DNA vaccines were successfully constructed and the antigen genes could be expressed effectively in vivo. The findings also demonstrated best synergistic effect of IL-2 with this protein which suggested that co-administration of cytokines with this antigen was a powerful method to enhance immunity by alleviating intestinal lesions, body w...Continue Reading

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Citations

Jan 1, 2014·Research in Veterinary Science·Guohua ChenXuepeng Cai
Dec 3, 2013·Indian Journal of Virology : an Official Organ of Indian Virological Society·Sami UllahNosheen Riaz
Jan 23, 2016·Veterinary Parasitology·ZhenChao ZhangXiangRui Li
Jul 17, 2015·Avian Pathology : Journal of the W.V.P.A·Jingwei HuangXiangrui Li
May 27, 2014·Research in Veterinary Science·Wenyan ShiXiaomin Zhao
Jul 24, 2015·Acta Parasitologica·Halina Wedrychowicz
Jun 25, 2010·Parasitology Research·Muhammad Ali A ShahXiangrui Li
Sep 11, 2018·The Onderstepoort Journal of Veterinary Research·Reza PasandidehMohammad Taghi Beigi Nassiri

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