Construction of Escherichia coli Whole-Cell Biosensors for Statin Efficacy and Production Test

Frontiers in Cell and Developmental Biology
Huanjie LiHuaiwei Liu

Abstract

Statins are widely used cholesterol-lowering drugs. Their potential application in anti-cancer treatment is also under investigation. The individual variance in statin response has been observed, which may be caused by the variation in human HMG-CoA reductase (hHMGR)-the inhibition target of statin drugs. Herein, we reported the design and construction of two Escherichia coli whole-cell biosensors. The first one is statin-efficacy testing sensor, which is composed of two separate modules: a hybrid mevalonate (MVA) pathway and a HMG-CoA sensing system. A truncated hHMGR was used as the key enzyme of the MVA pathway and a promiscuous transcription factor (TF) BsFapR was used as the HMG-CoA sensor. When hHMGR was inhibited by statins, HMG-CoA accumulated intracellularly and was sensed by BsFapR, which subsequently turned on its cognate promoter. This biosensor has the potential to be used as a "precision medicine" tool-selecting potent statin drugs for individual patients. The second one is a statin-production testing sensor, which is based on another promiscuous TF AraCM that can sense statins. This biosensor can be used in optimization of statin-producing strains. The prototypes of these two biosensors were successfully construc...Continue Reading

References

Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·L TaberneroC V Stauffacher
May 12, 2001·Science·E S Istvan, J Deisenhofer
Apr 26, 2003·Atherosclerosis. Supplements·Eva Istvan
Nov 9, 2004·Atherosclerosis. Supplements·Akira Endo
Jun 15, 2007·Lancet·Jane Armitage
Jun 26, 2010·Biopolymers·Chantel D Campbell, John C Vederas
Aug 2, 2013·Diabetes Care·Rafael BitzurDror Harats
Nov 29, 2013·ACS Synthetic Biology·Solvej SiedlerMichael Bott
Oct 24, 2014·Frontiers in Bioengineering and Biotechnology·Nathan GouldDimitris Papamichail
Nov 2, 2015·Applied Microbiology and Biotechnology·Regina Mahr, Julia Frunzke
Jan 14, 2017·Journal of the Royal Society of Medicine·Livio Garattini, Anna Padula
Jan 17, 2017·The American Journal of Medicine·Charles H HennekensMarc A Pfeffer
Feb 22, 2017·ACS Synthetic Biology·Yves BersetJan R van der Meer
Dec 1, 2017·Recent Patents on Anti-cancer Drug Discovery·Federica IannelliFrancesca Bruzzese
Jan 27, 2018·General Physiology and Biophysics·Jana SopkováErika Halašová
Aug 1, 2018·Analytical Chemistry·Mst Afroza KhatunYan Feng
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kyung-Jong WonSung-Hee Hwang

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