Construction of fibronectin conditional gene knock-out mice and the effect of fibronectin gene knockout on hematopoietic, biochemical and immune parameters in mice

PeerJ
Xiaohong YuanYuanzhong Chen

Abstract

Fibronectin (FN) is a multi-functional glycoprotein that primarily acts as a cell adhesion molecule and tethers cells to the extra cellular matrix. In order to clarify the effect of FN deficiency on hematopoiesis, biochemical and immune parameters in mice. We constructed a tamoxifen-induced conditional (cre-loxp system) fibronectin knock-out (FnKO) mouse model on a C57BL/6 background, and monitored their behavior, fertility, histological, hematopoietic, biochemical and immunological indices. We found that the Fn KO mice had reduced fertility, high platelet counts, smaller bone marrow megakaryocytes and looser attachment between the hepatocyte and vascular endothelial junctions compared to the wild type (WT) mice. In contrast, the behavior, hematological counts, serum biochemical indices and vital organ histology were similar in both Fn KO and WT mice. This model will greatly help in elucidating the role of FN in immune-related diseases in future.

References

Jan 1, 1985·Annual Review of Cell Biology·R Hynes
Sep 24, 2002·Journal of Cell Science·Roumen Pankov, Kenneth M Yamada
Feb 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Heyu NiDenisa D Wagner
Jul 6, 2004·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Guadalupe Ruiz MartínCristina Fernández
Aug 3, 2005·Matrix Biology : Journal of the International Society for Matrix Biology·Yong Mao, Jean E Schwarzbauer
Aug 6, 2008·The Journal of Pathology·E S WhiteA F Muro
Mar 21, 2012·The Journal of Cell Biology·Richard O Hynes
Apr 20, 2012·EMBO Molecular Medicine·Ina RohwedderReinhard Fässler
May 9, 2013·Circulation Research·Mathias H KonstandinMark A Sussman
Jul 24, 2018·PLoS Biology·Heena KumraDieter P Reinhardt
Feb 9, 2019·The Journal of Experimental Medicine·Alessandro MalaraAlessandra Balduini
Oct 10, 2019·Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University·Anna Lemańska-Perek, Barbara Adamik

❮ Previous
Next ❯

Methods Mentioned

BETA
gene knockout
PCR
transmission electron microscopy
ELISA
Electrophoresis
Flow cytometry

Software Mentioned

FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
Anna Lemańska-Perek, Barbara Adamik
Comptes rendus des séances de la Société de biologie et de ses filiales
J Labat-Robert, X Chevalier
The Journal of Biological Chemistry
Vasantha L KolachalaShanthi V Sitaraman
© 2021 Meta ULC. All rights reserved